Functional expression and regulation of eukaryotic cytochrome P450 enzymes in surrogate microbial cell factories

作     者：Pradeepraj Durairaj Shengying Li 

作者机构：State Key Laboratory of Microbial TechnologyShandong UniversityQingdao 266237ShandongChina Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdao 266237ShandongChina 

出 版 物：《Engineering Microbiology》 (工程微生物学（英文）)

年 卷 期：2022年第2卷第1期

页      面：17-34页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 081704[工学-应用化学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 0836[工学-生物工程] 0702[理学-物理学] 

基　　金：supported by the National Key Research and Develop-ment Program of China(2019YFA0706900) the National Natural Sci-ence Foundation of China(32025001 and 21472204) the Shandong Provincial Natural Science Foundation(ZR2019ZD20) 

主　　题：Cytochrome P450 enzymes Cytochrome P450 reductase Membrane-bound proteins N-terminal transmembrane domain Heterologous expression Microbial cell factories Redox partners Electron transfer 

摘      要：Cytochrome P450(CYP)enzymes play crucial roles during the evolution and diversification of ancestral monocel-lular eukaryotes into multicellular eukaryotic organisms due to their essential functionalities including catalysis of housekeeping biochemical reactions,synthesis of diverse metabolites,detoxification of xenobiotics,and con-tribution to environmental *** CYPs with versatile functionalities are undeniably regarded as promising biocatalysts with great potential for biotechnological,pharmaceutical and chemical industry ***,the modes of action and the challenges associated with these membrane-bound proteins have hampered the effective utilization of eukaryotic CYPs in a broader *** review is focused on comprehen-sive and consolidated approaches to address the core challenges in heterologous expression of membrane-bound eukaryotic CYPs in different surrogate microbial cell factories,aiming to provide key insights for better studies and applications of diverse eukaryotic CYPs in the *** also highlight the functional significance of the previously underrated cytochrome P450 reductases(CPRs)and provide a rational justification on the progression of CPR from auxiliary redox partner to function modulator in CYP catalysis.