Manipulated precipitation regulated carbon and phosphorus limitations of microbial metabolisms in a temperate grassland on the Loess Plateau,China

作     者：HAI Xuying LI Jiwei LIU Yulin WU Jianzhao LI Jianping SHANGGUAN Zhouping DENG Lei HAI Xuying;LI Jiwei;LIU Yulin;WU Jianzhao;LI Jianping;SHANGGUAN Zhouping;DENG Lei

作者机构：State Key Laboratory for Soil Erosion and Dryland Farming on the Loess PlateauInstitute of Soil and Water ConservationNorthwest A&F UniversityYangling 712100China Institute of Soil and Water ConservationChinese Academy of Science and Ministry of Water ResourcesYangling 712100China School of AgricultureNingxia UniversityYinchuan 750021China 

出 版 物：《Journal of Arid Land》 (干旱区科学（英文版）)

年 卷 期：2022年第14卷第10期

页      面：1109-1123页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0713[理学-生态学] 

基　　金：the National Natural Science Foundation of China(41730638) the Key Research and Development Program of Shaanxi Province,China(2021ZDLSF05-02) the Scientific and Technological Innovation Project of Shaanxi Forestry Academy of Sciences,China(SXLK2021-0206) the Funding of Special Support Plan of Young Talents Project in China(2021) the National Forestry and Grassland Administration in China(20201326015). 

主　　题：carbon use efficiency ecoenzymatic stoichiometry microbial metabolic limitations semi-arid ecosystems soil organic carbon 

摘      要：Manipulated precipitation patterns can profoundly influence the metabolism of soil microorganisms.However,the responses of soil organic carbon(SOC)and nutrient turnover to microbial metabolic limitation under changing precipitation conditions remain unclear in semi-arid ecosystems.This study measured the potential activities of enzymes associated with carbon(C:β-1,4-glucosidase(BG)andβ-D-cellobiosidase(CBH)),nitrogen(N:β-1,4-N-acetylglucosaminidase(NAG)and L-leucine aminopeptidase(LAP))and phosphorus(P:alkaline phosphatase(AP))acquisition,to quantify soil microbial metabolic limitations using enzymatic stoichiometry,and then identify the implications for soil microbial metabolic limitations and carbon use efficiency(CUE)under decreased precipitation by 50%(DP)and increased precipitation by 50%(IP)in a temperate grassland.The results showed that soil C and P were the major elements limiting soil microbial metabolism in temperate grasslands.There was a strong positive dependence between microbial C and P limitations under manipulated precipitation.Microbial metabolism limitation was promoted by DP treatment but reversed by IP treatment.Moreover,CUE was inhibited by DP treatment but promoted by IP treatment.Soil microbial metabolism limitation was mainly regulated by soil moisture and soil C,N,and P stoichiometry,followed by available nutrients(i.e.,NO^(-)_(3),NH^(+)_(4),and dissolved organic C)and microbial biomass(i.e.,MBC and MBN).Overall,these findings highlight the potential role of changing precipitation in regulating ecosystem C turnover by limiting microbial metabolism and CUE in temperate grassland ecosystems.