Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes

作     者：Yan Dai Meijia Huang Yujuan Xu Lixia Mu Jingyan Gao Hongbing Chen Zhihua Wu Anshu Yang Yong Wu Xin Li Yan Dai;Meijia Huang;Yujuan Xu;Lixia Mu;Jingyan Gao;Hongbing Chen;Zhihua Wu;Anshu Yang;Yong Wu;Xin Li

作者机构：State Key Laboratory of Food Science and TechnologyNanchang UniversityNanchang 330047China School of Food Science and TechnologyNanchang UniversityNanchang 330047China Sericultural and Agri-Food Research Institute Guangdong Academy of Agricultural SciencesKey Laboratory of Functional FoodsMinistry of Agriculture and Rural AffairsGuangdong Key Laboratory of Agricultural Products ProcessingGuangzhou 510610China Sino-German Joint Research InstituteNanchang UniversityNanchang 330047China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2023年第12卷第3期

页      面：774-782页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 083201[工学-食品科学] 

基　　金：supported by Special Project on the Integration of Industry,Education and Research of Guangdong Provine(2013B090600060) National Key R&D Program of China(2018YFC1604205) National Natural Science Foundation of China(31760431)。 

主　　题：Silkworm pupa Silkworm pupa peptides Enzymatic hydrolysis Allergenicity Immunization Animal model 

摘      要：Silkworm pupa is a nourishing food with high nutritional value,but its consumption has been greatly limited given its allergenicity.Enzyme hydrolytic technique is recognized as an effective method to reduce the allergenicity of protein.In this study,we aimed to investigate the effect of enzymolysis on the allergenicity of silkworm pupa.Crude silkworm pupa protein was extracted through alkali extraction and acid precipitation,which included 5 proteins with the molecular weights ranging from 34 kDa to 76 kDa,and silkworm pupa were then hydrolyzed by alkaline protease.The allergenicity of silkworm pupa protein and its enzymatic hydrolysates was evaluated by establishing BALB/c mice model,and the mice were immunized via intragastric gavage and intraperitoneal injection,respectively.The results indicated that the intraperitoneal inj ection immunization route induced more by detecting with antibodies,histamine and Th2-related cytokines.Moreover,mice treated with silkworm pupa protein peptide displayed no obvious allergic symptoms,indicating that enzyme hydrolytic technique could significantly reduce the allergenicity of silkworm pupa.