Contamination and browning in tissue culture of Platanus occidentalis L.

作     者：Tao Feng-jie Zhang Zhi-yi Zhou Jun Yao Na Wang Dong-mei 

作者机构：Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education Beijing Forestry University100083 R R. China 

出 版 物：《Forestry Studies in China》 (中国林学（英文版）)

年 卷 期：2007年第9卷第4期

页      面：279-282页

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：Beijing Forestry University Master Candidate Independent Research Fund  (05jj037) 

主　　题：platanus occidentalis L. tissue culture contamination browning 

摘      要：Twigs of 2-3-year-old Platanus occidentalis L. were used as experimental material to find the causes for the contamination and browning in the initial stages of tissue cultures. To compare the degree of browning of explants picked off from different growing seasons, the experimental material was excised from trees on each of the first ten days in January, March, May and July, 2006. The results indicated that the contamination and browning rates of the material cut off in January （14.2% and 30.6%, respectively） and March were somewhat lower than those in July. The pretreatment of soaking the explants in different anti-oxidants and absorbents at the same time could diminish some side effects. The pretreatment of using 10 g·L^-1 vitamin C reduced the contamination and brown- ing rate effectively. An orthogonal experiment showed that the optimal factor and level arrangement is 0.5 mg·L^-1BA, 2.0 g·L^-1 active carbon and 1.5 g·L^-1 PVP which resulted in a browning rate of only 16.5%. In general, sampling period, physical properties and pretreatment of explants are the main factors responsible for the contamination and browning of material in the initial stages of P. occidentalis tissue cultures.