Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay
Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay作者机构:Tianjin Key Laboratory of Food Science and HealthSchool of MedicineNankai UniversityTianjin 300071China College of Food Science and EngineeringTianjin University of Science and TechnologyTianjin 300457China
出 版 物:《Food Science and Human Wellness》 (食品科学与人类健康(英文))
年 卷 期:2023年第12卷第3期
页 面:745-754页
核心收录:
学科分类:0832[工学-食品科学与工程(可授工学、农学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 083201[工学-食品科学]
基 金:financially supported by the grants from the National Key R&D Program of China(No.2019YFC1605005)
主 题:Peanut allergen Ara h 3 Nanobody Sandwich ELISA Gly 1
摘 要:Peanut allergy is considered to be a major health issue with global *** date,no effective curative approach has been applied for the therapy of the anaphylaxis resulting from the peanut *** accurate and effective detection methods for the surveillance of allergens in food are still the primary strategies to avoid allergic *** this study,nanobodies(Nbs)derived from the Heavy-Chain only Antibodies(HCAbs)were selected against the general peanut protein extract through the unbiased strategy to facilitate the development of the sandwich ELISA for the detection and surveillance of peanut allergen *** target antigen of the selected Nb was identified as peanut allergen Ara h 3,and a cross-reaction was observed with the member of Gly 1 from the Ara h 3 *** applicability of the self-paired Nb P43 on the establishment of the immuno-assay was verified.A sandwich ELISA against peanut allergen was developed,which reached a linear range of 0.2-10.6μg/mL,and a limit of detection of 53.13 ng/mL.