Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay

作     者：Yaozhong Hu Chuan Zhang Jing Lin Yi Wang Sihao Wu Ying Sun Bowei Zhang Huan Lv Xuemeng Ji Yang Lu Shuo Wang Yaozhong Hu;Chuan Zhang;Jing Lin;Yi Wang;Sihao Wu;Ying Sun;Bowei Zhang;Huan Lü;Xuemeng Ji;Yang Lu;Shuo Wang

作者机构：Tianjin Key Laboratory of Food Science and HealthSchool of MedicineNankai UniversityTianjin 300071China College of Food Science and EngineeringTianjin University of Science and TechnologyTianjin 300457China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2023年第12卷第3期

页      面：745-754页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 083201[工学-食品科学] 

基　　金：financially supported by the grants from the National Key R&D Program of China(No.2019YFC1605005) 

主　　题：Peanut allergen Ara h 3 Nanobody Sandwich ELISA Gly 1 

摘      要：Peanut allergy is considered to be a major health issue with global *** date,no effective curative approach has been applied for the therapy of the anaphylaxis resulting from the peanut *** accurate and effective detection methods for the surveillance of allergens in food are still the primary strategies to avoid allergic *** this study,nanobodies(Nbs)derived from the Heavy-Chain only Antibodies(HCAbs)were selected against the general peanut protein extract through the unbiased strategy to facilitate the development of the sandwich ELISA for the detection and surveillance of peanut allergen *** target antigen of the selected Nb was identified as peanut allergen Ara h 3,and a cross-reaction was observed with the member of Gly 1 from the Ara h 3 *** applicability of the self-paired Nb P43 on the establishment of the immuno-assay was verified.A sandwich ELISA against peanut allergen was developed,which reached a linear range of 0.2-10.6μg/mL,and a limit of detection of 53.13 ng/mL.