Genome-wide association study revealed genetic variations of ABA sensitivity controlled by multiple stress-related genes in rice

作     者：Lei Peng Tingting Xie Zilong Guo Xiaokai Li Yu Chang Haifu Tu Shengchang Wang Nai Wu Yilong Yao Lizhong Xiong 

作者机构：National Key Laboratory of Crop Genetic ImprovementHubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China 

出 版 物：《Stress Biology》 (逆境生物学（英文）)

年 卷 期：2021年第1卷第1期

页      面：120-131页

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by grants from the National Natural Science Foundation of China(31930080 31821005). 

主　　题：ABA sensitivity Seed germination Stress response Rice 

摘      要：Abscisic acid(ABA)is a critical phytohormone that regulates multiple physiological processes including plant growth and stress tolerance.The core ABA signaling pathway has been well established,but genetic variations mediating ABA responses remain largely unknown.In this study,we performed genome-wide association study(GWAS)to identify loci and genes associated with ABA sensitivity(reflected by seed germination inhibition by ABA)in a panel of 425 rice accessions.The seed germination assay revealed that Aus and indica rice had stronger ABA sensitivity than japonica rice.A total of 48 non-redundant association loci were detected in the indica subpopulation and whole population,and 386 genes in these loci were responsive to ABA or abiotic stresses.Eight association loci were overlapped with previously reported loci for yield under drought stress or for droughtindicative image traits.Haplotype analyses of important candidate genes such as OsSAPK6,a key component in the ABA signaling core,were performed to identify key SNPs/InDels that may affect gene functions through promoter activity regulation,amino acid variation,or gene splicing.These results provide insights into the genetic basis of ABA sensitivity related to stress responses.