The Concentration of Hydrogen Peroxide Generated during Aggregation of a-Synuclein in vitro Is Lower than 5 nmol/L

作     者：吉丽娜 李洪涛 罗晓莹 张峰 胡红雨 胡钧 

作者机构：Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sci-ences Chinese Academy of Sciences Shanghai 200031 China Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 ChinaBio-X Research Center Shanghai Jiaotong University Shanghai 200030 China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2004年第22卷第12期

页      面：1440-1443页

核心收录：

学科分类：07[理学] 070305[理学-高分子化学与物理] 0703[理学-化学] 

基　　金：Project supported by the National Natural Science Foundation of China (No. 30070165) Science & Technology Committee of Shanghai (Nos. 0159NM078 03JC14081). 

主　　题：a-synuclein hydrogen peroxide aggregation cytotoxicity 

摘      要：Using a fluorometric method with a detection limit of 5 nmol/L, here it is reported that albeit positive results were got from bovine serum albumin (BSA) and chicken ovalbumin (OVA) as published in literature, no detectable amount of hydrogen peroxide (H2O2) was generated during a-synuclein (a-Syn) aggregation in vitro even in the presence of transition metal ions Cu(II) or Fe(III). The results suggest that the concentration of H2O2 generated during aggregation of a-Syn in vitro be lower than 5 nmol/L beyond the detection limit of the adopted method and it is far too poor to be responsible for the cytotoxicity of a-Syn aggregates, thus allowing people to extensively elu-cidate the mechanism underlying neurotoxicities of the aggregates formed by some amyloidogenic proteins.