Exploring the potential for an evolutionarily conserved role of the taste 1 receptor gene family in gut sensing mechanisms of fish

作     者：Anna Rita Angotzi Esther Leal Sara Puchol José M. Cerdá-Reverter Sofia Morais Anna Rita Angotzi;Esther Leal;Sara Puchol;José M.Cerdá-Reverter;Sofia Morais

作者机构：Department of Fish Physiology and BiotechnologyInstituto de Acuicultura de Torre de la SalIATS-CSICTorre la Sal s/nRibera de Cabanes12595 CastellonSpain Lucta S.A.Innovation DivisionAnimal Science UnitUAB Research Park08193 BellaterraSpain 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2022年第11卷第4期

页      面：293-308页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：covered by the National Research Agency(AEI Spain)(grant number:PID2019-103969RB-C33)to Jos e M.Cerd a-Reverter 

主　　题：Taste 1 receptor Fish larvae Gut nutrient sensing Gut peptides G(i)alpha protein subunits 1 and 2 

摘      要：In this study,we investigated the transcriptional spatio-temporal dynamics of the taste 1 receptor(T1R)gene family repertoire in seabream(Sparus aurata[sa]),during larval ontogeny and in adult *** early larval development,sa T1R expression arises heterochronously,*** extraoral taste-related perception in the gastrointestinal tract(GIT)anticipates first exogenous feeding(at 9 days post hatching[dph]),followed by the buccal/intraoral perception from 14 dph onwards,supporting the hypothesis that the early onset of the molecular machinery underlying sa T1R expression in the GIT is not induced by food but rather genetically *** adulthood,we characterized the expression patterns of sa T1R within specific tissues(n=4)distributed in oropharingeal,GIT and brain regions substantiating their functional versatility as chemosensory signaling players to a variety of biological functions beyond oral taste ***,we provided for the first time direct evidences in fish for m RNA coexpression of a subset of sa T1R genes(mostly sa T1R3,*** common subunit of the heterodimeric T1R complexes for the detection of“sweetand“umamisubstances),with the selected gut peptides ghrelin(ghr),cholecystokinin(cck),hormone peptide yy(pyy)and proglucagon(pg).Each peptide defines the enteroendocrine cells(ECCs)identity,and establishes on morphological basis,a direct link for T1R chemosensing in the regulation of fish digestive ***,we analyzed the spatial gene expression patterns of 2 taste signaling components functionally homologous to the mammalian G(i)a subunit gustducin,namely sa G(i)a1 and sa G(i)a2,and demonstrated their co-localization with the sa T1R3in EECs,thus validating their direct involvement in taste-like transduction mechanisms of the fish *** conclusion,data provide new insights in the evolutionary conservation of gut sensing in fish suggesting a conserved role for nutrient sensors modulating entero-endocrine secretion.