RNA-seq analysis of protective effect of epicatechin gallate on H_(2)O_(2)-induced oxidative injury in PC12 cells
作者机构：School of Life Science and BiopharmaceuticsShenyang Pharmaceutical UniversityShenyang 110016China Faculty of Functional Food and WineShenyang Pharmaceutical UniversityShenyang 110016China
出 版 物：《Journal of Polyphenols》 (多酚杂志（英文）)
年 卷 期：2022年第4卷第1期
页 面：1-16页
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
主 题：epicatechin gallate H_(2)O_(2) oxidative injury RNA-seq cell cycle
摘 要：Epicatechin gallate(ECG)is one of the polyphenolic compounds and has attracted much attention due to its various *** this study,the neuroprotective eff ect of ECG against H_(2)O_(2)-induced oxidative injury in PC12 cells as well as the possible mechanisms were *** viability was determined by MTT *** differentially expressed genes(DEGs),GO enrichment,and KEGG enrichment were analyzed to explore the mechanism of ECG against H_(2)O_(2)-induced oxidative injury by using the RNA-seq ***,the change in the cell cycle was analyzed by fl ow cytometry.H_(2)O_(2)(400-1200μmol/L)inhibited the cell viability in a concentration-dependent ***(6-150μmol/L)eff ectively attenuated the H_(2)O_(2)-induced decrease in cell ***-seq analysis showed that ECG regulated 1058 coexpressed *** enrichment analysis showed that the cellular component was the dominant group after ECG *** analysis showed that the cell cycle,fanconi anemia pathway,and homologous recombination were the important pathways for ECG in improving H_(2)O_(2)-induced oxidative injury and 28 coexpressed DEGs in the cell cycle pathway were ***,cell cycle analysis also proved that ECG improved H_(2)O_(2)-induced cell cycle arrest in the G2/M *** present study demonstrated that ECG attenuated H_(2)O_(2)-induced neurologic oxidative damage by multiple modulatory mechanisms at the molecular transcription *** fi ndings provide new insights for further study of the molecular mechanism of the neuroprotection of ECG.
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