Ginsenoside Rb1 attenuates lipopolysaccharide-induced chronic neuroinflammation in mice by tuning glial cell polarization

作     者：Yushu Liu Juan Li Xi Wang Ying Liu Chao Zhang Hlupheka Chabalala Minke Tang Yushu Liu;Juan Li;Xi Wang;Ying Liu;Chao Zhang;Hlupheka Chabalala;Minke Tang

作者机构：School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijing102488China IK-Based Technology InnovationDepartment of Science and TechnologyPretoria0001South Africa 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2022年第9卷第4期

页      面：383-391页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This research was supported by the grant“Prevention and Treatment of Major Diseases with Chinese Medicine from the National Administration of Traditional Chinese Medicine(GZYYGJ2021047)” the“High-end Experts Support Program from the Ministry of Science and Technology(DL 2021110001L)” the“Basic Research Funds from the Ministry of Education 1000061223731)” 

主　　题：Astrocytes Chronic neuroinflammation Ginsenoside Rb1 Glial cell polarization Lipopolysaccharide Microglia Neurodegenerative diseases 

摘      要：Objective:To evaluate whether ginsenoside Rb1(Rb1) can attenuate lipopolysaccharide(LPS)-induced chronic neuroinflammation in mice and to explore its relationship with glial cell ***:Intraperitoneal injection with an escalating dose of LPS was used to establish a chronic neuroinflammation model in *** LPS was initiated,10 or 20 mg/kg Rbl,or sterile saline,was administered for 14 consecutive *** field test and beam walking test were used to monitor the changes in *** concentration of cytokines in the serum and brain were used to monitor the systemic inflammation and neuroinflammation,*** specific to each glial cell phenotype were used to investigate glial cell ***:Mice in the LPS group had reduced spontaneous activities and impaired beam walking *** obviously eased LPS-induced behavior distu *** the levels of serum cytokines,both tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were significantly increased,while interleukin-10(IL-10) and transforming growth factor β(TGF-β) remarkably decreased after LPS treatment(all P .001).Rb1 treatment significantly attenuated LPS-induced serum cytokine changes(all P .05).The results of quantitative polymerase chain reaction and western blotting showed that the mRNA and protein expression levels of TNF-α and complement component 3(C3) in the brain were significantly increased after LPS treatment(all P.01).Rbl treatment significantly inhibited LPS-induced inflammation in the brain(all P .05).Glial cell polarization analysis showed that M1 and M2 microglia,and A1 astrocytes increased following LPS treatment,while A2 astrocytes ***1 treatment reduced M1 and M2 microglia,and A1 astrocytes,and significantly increased A2 ***:Rb1 can attenuate chronic neuroinflammation induced by LPS in mice,which may be partially attributable to its fine tuning of microglia and astrocyte ***1 has potentia