An emerging role of vitamin D_(3) in amino acid absorption in different intestinal segments of on-growing grass carp(Ctenopharyngodon idella)

作     者：Yao Zhang Chao-Nan Li Wei-Dan Jiang Pei Wu Yang Liu Sheng-Yao Kuang Ling Tang Shu-Wei Li Xiao-Wan Jin Hong-Mei Ren Xiao-Qiu Zhou Lin Feng Yao Zhang;Chao-Nan Li;Wei-Dan Jiang;Pei Wu;Yang Liu;Sheng-Yao Kuang;Ling Tang;Shu-Wei Li;Xiao-Wan Jin;Hong-Mei Ren;Xiao-Qiu Zhou;Lin Feng

作者机构：Animal Nutrition InstituteSichuan Agricultural UniversityChengduChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan ProvinceSichuan Agricultural UniversityChengduChina Key Laboratory for Animal Disease-resistant Nutrition of China Ministry of EducationSichuan Agricultural UniversityChengduChina Sichuan Animal Science AcademySichuan Animtech Feed Co.LtdChengduChina Animal Breeding and Genetics Key Laboratory of Sichuan ProvinceAnimal Nutrition InstituteSichuan Academy of Animal ScienceChengduChina 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2022年第10卷第3期

页      面：305-318页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：National Key R&D Program of China(2019YFD0900200,2018YFD0900400) National Natural Science Foundation of China for Outstanding Youth Science Foundation(31922086) Young Top-Notch Talent Support Program,China Agriculture Research System of MOF and MARA(CARS-45) Sichuan Science and Technology Program(2019YFN0036) 

主　　题：Vitamin D_(3) Intestinal segment Intestinal absorption Amino acid transporter Peptide transporter TOR signaling 

摘      要：Vitamin D_(3)(VD_(3)),an essential nutrient for animals,has been demonstrated to stimulate the uptake of certain amino ***,the role of VD_(3) in the intestine,the primary site for digestion and absorption of nutrients,remains poorly ***,the grass carp(Ctenopharyngodon idella)was studied to assess the influence of different doses of VD_(3)(15.2,364.3,782.5,1,167.9,1,573.8,and 1,980.1 IU/kg)on growth performance,intestinal morphology,digestive absorption,amino acid transport,and potential signaling molecule levels in a feeding *** a result,dietary VD_(3) improved growth performance,intestinal structure,and digestive and brush border enzyme ***,most intestinal free amino acids and their transporters were upregulated after VD_(3) intake,except for Ala,Lys,Asp,Leu,solute carrier(SLC)7A7,SLC1A5,and SLC1A3 mRNA in different segments,Leu and SLC6A14 mRNA in the proximal intestine,and SLC7A5 mRNA in the mid and distal *** the crucial target of rapamycin(TOR)signal pathway of amino acid transport,the gene and protein expression of TOR,S6 kinase 1,and activating transcription factor 4 were elevated,whereas 4E-binding protein 1 was decreased,further suggesting an advanced amino acid absorption capacity in the fish due to VD_(3) *** on percentage weight gain,feed efficiency,and trypsin activity,the VD_(3) requirements of on-growing grass carp were estimated to be 968.33,1,005.00,and 1,166.67 IU/kg,*** findings provide novel recommendations for VD_(3) supplementation to promote digestion and absorption capacities of fish,contributing to the overall productivity of aquaculture.