Development of powdery mildew resistant derivatives of wheat variety Fielder for use in genetic transformation

作     者：Xiaona Liang Xiaomin Bie Yuliang Qiu Ke Wang Zujun Yang Yuqi Jia Zhiying Xu Mei Yu Lipu Du Zhishan Lin Xingguo Ye Xiaona Liang;Xiaomin Bie;Yuliang Qiu;Ke Wang;Zujun Yang;Yuqi Jia;Zhiying Xu;Mei Yu;Lipu Du;Zhishan Lin;Xingguo Ye

作者机构：Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China College of Life ScienceShandong Agricultural UniversityTai’an 271018ShandongChina School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengdu 611731SichuanChina College of AgricultureYangtze UniversityJingzhou 434025HubeiChina National Key Facility of Crop Gene Resources and Genetic ImprovementBeijing 100081China Key Laboratory of Ministry of Agriculture and Rural Affairs of China for Biology and Genetic Breeding of Triticeae CropsBeijing 100081China 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2023年第11卷第2期

页      面：573-583页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31971945) 

主　　题：Genotype improvement Plant regeneration ability Transformation efficiency Triticum aestivum 

摘      要：Genetic transformation is widely used to improve target traits and to study gene function in ***,transformation efficiency depends on the physiological status of the recipient genotype and that is affected by several factors including powdery mildew(PM)*** widely used recipient variety Fielder is very susceptible to ***,it would be beneficial to develop PM resistant derivatives with high regeneration ability for use in genetic *** the present study PM resistant lines CB037 and Pm97033 carrying genes Pm21 and PmV,respectively,were backcrossed to Fielder with selection for PM *** lines,NT89,NT90,NT154,and WT48 with Pm21 and line FL347 with PmV were developed,identified by molecular markers and genomic in situ hybridization(GISH)or fluorescent in situ hybridization(FISH),and further subjected to detailed assessment of agronomic traits and regeneration ability following genetic transformation *** FL347,WT48,NT89 and NT154 assessed as being equal to,or superior,to Fielder in regeneration and transformation ability are recommended as suitable materials for the replacement of Fielder for wheat gene transfer and genome editing study.