Comparison of 16S rRNA Gene Primers on Studying Microbial Community Composition in Bottom Water and Sediment of Artificial Reefs in Laoshan Bay, China

作     者：FANG Guangjie YU Haolin SHENG Huaxiang TANG Yanli LIANG Zhenlin FANG Guangjie;YU Haolin;SHENG Huaxiang;TANG Yanli;LIANG Zhenlin

作者机构：Fisheries CollegeOcean University of ChinaQingdao 266003China Marine CollegeShandong UniversityWeihai 264200China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2022年第21卷第5期

页      面：1313-1322页

核心收录：

学科分类：090801[农学-水产养殖] 082803[工学-农业生物环境与能源工程] 0908[农学-水产] 08[工学] 0828[工学-农业工程] 09[农学] 

基　　金：funded by the Project of National Key R&D Program of China(No.2019YFD0901302) 

主　　题：16S rRNA hypervariable region ARs bacteria archaea 

摘      要：Marine microorganisms are indispensable regulators of nutrient cycling and energy flow,which are crucial for artificial reefs(ARs)***,little is known about the microbial communities in the bottom water and sediment of *** of microbial diversities have greatly advanced due to the development of high-throughput sequencing technologies,whereas the results may vary significantly due to the primers’*** study investigated the influences of two 16S ribosomal RNA gene primer choices(V4 and V3-V4)on microbial community compositions and *** results showed that the taxonomic assignment de-tected by primer V3-V4 was higher compared with that obtained by primer V4,whereas microbial community compositions had strong correlations between the two *** beta diversities of ARs can be uncovered by both primers,but the relation-ships between communities and environmental parameters were *** performances of the two primers for water samples were highly consistent,but the inconformity was evident for sediment *** the relatively lower taxonomic classification of primer V4 for sediment samples,primer V3-V4 was recommended for later *** the development and advancement of ARs in China,our findings provide a meaningful reference for ecologists focusing on the microbial diversities and ecological functions of these artificial habitats in the future.