Construction of a high-density mutant population of Chinese cabbage facilitates the genetic dissection of agronomic traits

作     者：Xiaoxue Sun Xing Li Yin Lu Shan Wang Xiaomeng Zhang Kang Zhang Xiangjie Su Mengyang Liu Daling Feng Shuangxia Luo Aixia Gu Yu Fu Xueping Chen Shuxin Xuan Yanhua Wang Donghui Xu Shumin Chen Wei Ma Shuxing Shen Feng Cheng Jianjun Zhao Xiaoxue Sun;Xing Li;Yin Lu;Shan Wang;Xiaomeng Zhang;Kang Zhang;Xiangjie Su;Mengyang Liu;Daling Feng;Shuangxia Luo;Aixia Gu;Yu Fu;Xueping Chen;Shuxin Xuan;Yanhua Wang;Donghui Xu;Shumin Chen;Wei Ma;Shuxing Shen;Feng Cheng;Jianjun Zhao

作者机构：State Key Laboratory of North China Crop Improvement and RegulationKey Laboratory of Vegetable Germplasm Innovation and Utilization of HebeiCollaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaoding 071000China Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural AffairsSino-Dutch Joint Laboratory of Horticultural GenomicsBeijing 271000China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2022年第15卷第5期

页      面：913-924页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by the Innovative Research Group Project of Hebei Natural Science Foundation(grant no.C2020204111) the National Natural Science Foundation of China(grant nos.32002054,31902005,31930098,31972411,31722048,31872947) the International Science and Technology Cooperation base Special Project of Hebei(grant no.20592901D) Key R&D Project of Hebei(grant no.21326344D) the Natural Science Foundation of Hebei(grant nos.C2020204122,C2021204163) the Introducing Foreign Intellectual Project of Hebei(grant no.2019YX023A) the Post doctoral Science Foundation of China(grant no.2020M680901) the Science and Technology Research Project of University in Hebei(grant no.BJ2019020) the Post-graduate's Innovation Fund Project of Hebei(grant no.CXZZBS2017069) the State Key Laboratory of North China Crop Improvement and Regulation(NCCIR20202KF-2). 

主　　题：Chinese cabbage EMS morphological variations whole-genome sequencing functional genomics 

摘      要：Chinese cabbage(Brassica rapa ssp.pekinensis)is an economically important vegetable crop throughout the world,especially in Asia.High-quality genome sequences are available for Chinese cabbage,but gene functional studies remain challenging.To promote functional genomic studies of Chinese cabbage,we generated an ethyl methane sulfonate(EMS)mutant population of~8000 M_(2) plants using the double haploid inbred line A03 as the parent.The genome of A03 was sequenced and used as a reference for high-throughput functional characterization of gene mutations at the whole-genome level.A total of 300 M_(2) to M_(5) EMS mutants were phenotypically screened and then sequenced,revealing 750629 SNPs and 46272 InDel mutations that cover 98.27%of all predicted genes in the A03 genome.A forward-genetics approach was successfully used to identify two genes with chloroplast-related functions that are responsible for the yellow leaf mutant trait.A reverse-genetics approach was also used to identify associations between mutations in five genes of the glucosinolate biosynthetic pathway and variations in glucosinolate content of the mutant plants.In addition,we built the Chinese cabbage EMS mutation database(CCEMD,***/EMSmutation/home)to increase the usability of this mutant population resource.In summary,we performed large-scale screening of a heading Chinese cabbage EMS mutant collection at the phenotypic and genotypic levels,which will facilitate gene mining of Chinese cabbage and might also be useful for the study of other Brassica crops.