Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma

作     者：Yu Huan Xiu-Quan Wu Tao Chen Ya-Nan Dou Bo Jia Xin He Dong-Yu Wei Zhou Fei Fei Fei Yu Huan;Xiu-Quan Wu;Tao Chen;Ya-Nan Dou;Bo Jia;Xin He;Dong-Yu Wei;Zhou Fei;Fei Fei

作者机构：Department of NeurosurgeryXijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina Center of Clinical Aerospace MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Aviation MedicineXijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of OphthalmologyXijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2023年第18卷第4期

页      面：922-928页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100212[医学-眼科学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81600738 the Youth Development Project of Air Force Medical University,No.21QNPY072(both to FF)。 

主　　题：Beagle dogs blunt ocular trauma gliosis M1 microglia Müller cells necroptosis neuroinflammation retinal ganglion cells retinal injury weight drop injury 

摘      要：Retinal injury after blunt ocular trauma may directly affect prognosis and lead to vision loss.To investigate the pathological changes and molecular mechanisms involved in retinal injury after blunt ocular trauma,we established a weight drop injury model of blunt ocular trauma in male Beagle dogs.Hematoxylin-eosin staining,immunofluorescence staining,western blotting,and TUNEL assays were performed to investigate retinal injury within 14 days after blunt ocular trauma.Compared with the control group,the thicknesses of the inner and outer nuclear layers,as well as the number of retinal ganglion cells,gradually decreased within 14 days after injury.The number of bipolar cells in the inner nuclear layer began to decrease 1 day after injury,while the numbers of cholinergic and amacrine cells in the inner nuclear layer did not decrease until 7 days after injury.Moreover,retinal cell necroptosis increased with time after injury;it progressed from the ganglion cell layer to the outer nuclear layer.Visual electrophysiological findings indicated that visual impairment began on the first day after injury and worsened over time.Additionally,blunt ocular trauma induced nerve regeneration and Müller glial hyperplasia;it also resulted in the recruitment of microglia to the retina and polarization of those microglia to the M1 phenotype.These findings suggest that necroptosis plays an important role in exacerbating retinal injury after blunt ocular trauma via gliosis and neuroinflammation.Such a role has important implications for the development of therapeutic strategies.