Effects of Granule Dendrobii on chronic atrophic gastritis induced by N-methyl-N'-nitro-N-nitrosoguanidine in rats

作     者：Yue Wu Yu Li Xiao-Ming Jin Guan-Hai Dai Xuan Chen Ye-Ling Tong Ze-Ming Ren Yu Chen Xiao-MinXue Ren-Zhao Wu 

作者机构：First College of Clinical MedicineZhejiang Chinese Medical UniversityHangzhou 310052Zhejiang ProvinceChina Institute of Basic MedicineZhejiang Academy of Traditional Chinese MedicineHangzhou 310007Zhejiang ProvinceChina College of Basic MedicineZhejiang Chinese Medical UniversityHangzhou 310052Zhejiang ProvinceChina Stark Neuroscience Research Institute&Department of AnatomyCell Biology and PhysiologyIndiana University School of MedicineIndianapolisIN 46202United States Department of Experimental AnimalsZhejiang Academy of Traditional Chinese MedicineHangzhou 310007Zhejiang ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2022年第28卷第32期

页      面：4668-4679页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Science and Technology Department of Zhejiang Province,No.LGF19H280008 the Provincial Natural Science Foundation of Zhejiang Province,No.LY15H280011 

主　　题：Chronic atrophic gastritis Granule Dendrobii N-methyl-N'-nitro-N-nitrosoguanidine Intestinal metaplasia 

摘      要：BACKGROUND Dendrobium officinale is an herb of Traditional Chinese Medicine(TCM)commonly used for treating stomach *** formula of Granule Dendrobii(GD)consists of Dendrobium officinale and American Ginseng(Radix Panacis quinquefolii),and is a potent TCM product in *** treatment with GD can promote gastric acid secretion and alleviate gastric gland atrophy in chronic atrophic gastritis(CAG)requires *** To determine the effect of GD treatment on CAG and its potential cellular *** A CAG model was induced by feeding rats N-methyl-N -nitro-N-nitrosoguanidine(MNNG)for 12 *** oral administration of low,moderate,and high doses of GD in CAG rats for 8 wk,its effects on body weight,gastric mucosa histology,mucosal atrophy,intestinal metaplasia,immunohistochemical staining of proliferating cell nuclear antigen(PCNA)and B-cell lymphoma-2,and hemoglobin and red blood cells were *** The body weights of MNNG-induced CAG model rats before treatment(143.5±14.26 g)were significantly lower than that of healthy rats(220.2±31.20 g,P0.01).At the 8th week of treatment,the body weights of rats in the low-,moderate-,and high-dose groups of GD(220.1±36.62 g)were significantly higher than those in the untreated group(173.3±28.09 g,all P0.01).The level of inflammation in gastric tissue of the high-dose group(1.68±0.54)was significantly reduced(P0.01)compared with that of the untreated group(3.00±0.00,P0.05).The number and thickness of gastric glands in the high-dose group(31.50±6.07/mm,306.4±49.32μm)were significantly higher than those in the untreated group(26.86±6.41/mm,244.3±51.82μm,respectively,P0.01 and P0.05),indicating improved atrophy of gastric *** areas of intestinal metaplasia were significantly lower in the high-dose group(1.74%±1.13%),medium-dose group(1.81%±0.66%)and low-dose group(2.36%±1.08%)than in the untreated group(3.91%±0.96%,all P0.01).The expression of PCNA in high-dose group was significantly