SARS-CoV-2 Epitopes following Infection and Vaccination Overlap Known Neutralizing Antibody Sites
作者机构：Center for Cllular and Molecular DiagnosticsTulane University Schoo of Medicine1430 Tulane AveNew OrleansLA 70112USA Department of Biochemisty and Molecular BiologyTulane University School of Medicine1430 Tulane AveNew OrleansLA 70112USA Beijing Key Laboratory for Forest Pest ControlBejjing Foresiry UniersilyBeijing 10083.China State Key Laboratory of ProteommicsBeijing Protcome Resarch CenterNattional Center for Protein Sciences-Beiing(PHOENIX Center)Beijing Institute of LijfeomicsBeijing 102206China Hayward Genetis CenerDepartment of PediatricsTulane Universily School of Medicine1430 Tulane AveNew OrleansLA 70112USA Department of PediatricsTulane Uhriversity School of Medicine1430 Ttulane AveNew OrleansLA 70112USA Infectious Disease DeparimentOchsner Clinic FoundationNew OrleansLA 70121USA Universty of Texas Souhwestern Medical Cener5323 Harry Himes BlvdDallasTX 75390USA Department of Microbiology Imruolog Tulane Untiversity School of Medicine1430 Tulane AveNew OrleansLA 70112USA Division of MicrobiologyTulane National Primate Research Center18703 Three Rivers RoadCovingtonLA 70433USA
出 版 物：《Research》 (研究（英文）)
年 卷 期：2022年第2022卷第2期
页 面：71-84页
核心收录：
学科分类：0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 100401[医学-流行病与卫生统计学] 10[医学]
基 金：supported by the Department of Defense(grant number W8IxwH19i0926) National Institute of Allergy and Infectious Diseases contract(grant number_HHSN2722017000331) National Institute of Child Health and Human Development grant(grant numbers R01HD090927 and R01HDi03511) National Center for Research Resources and the Ofice of Research Infrastructure Programs(grant numbers OD011104).
摘 要：Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites,but can be laborious.Herein,we employed peptide microarrays to map linear peptide epitopes(LPEs)recognized following SARS-CoV-2 infetion and vaccination.LPEs detected by nonhuman primate(NHP)and patient IgMs after SARS-CoV-2 infection extensively overlapped,localized to functionally important virus regions,and aligned with reported neutralizing antibody binding sies.Similar LPE overlap occurred atfter infection and vaccination,with LPE clusters specifc to each stimulus,where strong and conserved LPEs mapping to sites known or likely to inhibit spike protein function.Vaccine-specifc LPEs tended to map to sites known or likely to be afected by structural changes induced by the proline substitutions in the mRNA vaccine s S protein.Mapping LPEs to regions of known functional importance in this manner may acelerate vaccine evaluation and discovery of targets for sile secific therapeutic interventions.
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