Liquid crystal-based optical aptasensor for the sensitive and selective detection of Gram-negative bacteria

作     者：Mengjun Liu Minmin Zhang Jiamei Chen Ruizhi Yang Zhaofan Huang Zhenping Liu Na Li Lingling Shui 

作者机构：Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and DevicesSchool of Information and Optoelectronic Science and EngineeringSouth China Normal UniversityGuangzhou 510006China Joint Laboratory of Optofluidic Technology and SystemsNational Center for International Research on Green OptoelectronicsSouth China Academy of Advanced OptoelectronicsSouth China Normal UniversityGuangzhou 510006China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2022年第65卷第10期

页      面：2023-2030页

核心收录：

学科分类：07[理学] 080202[工学-机械电子工程] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0802[工学-机械工程] 0702[理学-物理学] 

基　　金：supported by Guangdong Province Basic and Applied Basic Research Foundation (2021A1515110236) the National Natural Science Foundation of China (62004070) the Science and Technology Program of Guangzhou (2019050001,202201010248) the Youth Innovation Project of Guangdong Education Department(2020KQNCX018) the Young Scholar Foundation of South China Normal University (21KJ08) the “Climbing Program” Special Funds of Guangdong Province (pdjh2022b0133) partially supported by the Science and Technology Planning Project of Guangdong Province (2020B1212060067) 

主　　题：liquid crystal molecular arrangement optical sensor aptamer Gram-negative bacteria 

摘      要：The sensitive and differential detection of Gram-negative bacteria is essential in food processing,environmental monitoring,and the daily chemical ***,we propose and validate a liquid crystal(LC)-based aptasensor for the ultrasensitive detection of Escherichia coli(***),a model of Gram-negative *** nematic liquid crystal of 4-cyano-4’-pentylbiphenyl(5 CB) molecules can be orderly or disorderly arranged at the LC-aqueous interface via different stimuli,causing changes in optical texture due to *** schlieren texture is observed when a mixture solution of aptamer and hexadecyl trimethyl ammonium bromide(CTAB) is dripped onto the segmented LC films on a copper *** specific binding of aptamers with target bacteria biomarkers liberates the CTAB molecules,which then self-assemble at the LC-aqueous interface to induce the vertical alignment of *** optical transition from bright to dark is therefore achieved via the LC molecular orientation and serves as an *** the prominent affinity and specificity of the aptamer,the established sensitive and selective *** assay shows an ultralow detection limit of 27 cfu/*** prepared aptasensor can also be applied for the sensitive and selective determination of *** in fruit juice,soft drink,and cosmetic products,and shows great promise for the on-site detection of Gram-negative bacteria with high sensitivity and specificity for environmental monitoring,food safety assessment,and household chemical inspection.