Ultrasound-visualized nanocarriers with siRNA for targeted inhibition of M2-like TAM polarization to enhance photothermal therapy in NSCLC

作     者：Wenhao Lv Chen Xu Hao Wu Yangyang Zhu Ozioma Udochukwu Akakuru Hui Du Fang Nie Aiguo Wu Juan Li Wenhao Lv;Chen Xu;Hao Wu;Yangyang Zhu;Ozioma Udochukwu Akakuru;Hui Du;Fang Nie;Aiguo Wu;Juan Li

作者机构：Ultrasound Medical CenterLanzhou University Second HospitalGansu Province Clinical Research Center for UltrasonographyGansu Province Medical Engineering Research Center for Intelligence UltrasoundLanzhou 730000China Cixi Institute of Biomedical EngineeringInternational Cooperation Base of Biomedical Materials Technology and ApplicationChinese Academy of Sciences(CAS)Key Laboratory of Magnetic Materials and DevicesZhejiang Engineering Research Center for Biomedical MaterialsNingbo Institute of Materials Technology and EngineeringCASNingbo 315201China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2023年第16卷第1期

页      面：882-893页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0901[农学-作物学] 100214[医学-肿瘤学] 0836[工学-生物工程] 10[医学] 

基　　金：This work was supported by the Natural Science Foundation of China(Nos.81873898,81960316,81871411,32011530115,and 32025021) the Science&Technology Bureau of Ningbo City(Nos.2020Z094 and 2021Z072) the Excellent Member of Youth Innovation Promotion Association Foundation of CAS(No.Y2021079) the Ningbo 3315 Innovative Teams Program,China(No.2019A-14-C) 

主　　题：gold-based nanocarrier small interfering RNA(siRNA)delivery photothermal therapy M2-like tumor-associated macrophages(TAMs) contrast-enhanced ultrasound imaging non-small cell lung cancer(NSCLC) 

摘      要：Photothermal therapy(PTT)has received a lot of attention as a promising strategy for eliminating tumors ***,the unavoidable inflammatory response during the treatment might result in a high concentration of M2-like tumor-associated macrophages(TAMs),increasing the risk of tumor recurrence and *** address this problem,gold-based nanocarriers(PGMP-small interfering RNA(siRNA)nanoparticles(NPs))containing STAT6siRNA,that inhibited M2-like TAM polarization,were designed and investigated for PTT and gene therapy of non-small cell lung cancer(NSCLC).In an NSCLC model,the nanocarriers demonstrated excellent siRNA delivery ability and a high gene transfection rate of up to 90%in macrophages,thus inhibiting the polarization of about 87%of M2-like TAMs and effectively suppressing the invasion and metastasis of ***,the unique gold nanosphere structure offered improved PTT and contrast-enhanced ultrasound imaging,thus contributing to the efficient elimination and real-time monitoring of the tumor *** nanocarriers with combined gene and photothermal therapeutic capabilities improved the efficacy of single-modality treatment,and showed the potential to inhibit cancer cell recurrence and metastasis to ultimately cure NSCLC.