Simultaneous Determination by HPLC-UV Vis of Tartrazine and Sunset Yellow in Soft Drinks Sold in Benin

作     者：Janvier Engelbert Agbokponto André Philippe S. Kpaibe Loconon Achille Yemoa Assogba Gabin Assanhou Habib Ganfon Gildas Komenan Gbassi Michèle Aké N’cho Christoph Amin Janvier Engelbert Agbokponto;André Philippe S. Kpaibe;Loconon Achille Yemoa;Assogba Gabin Assanhou;Habib Ganfon;Gildas Komenan Gbassi;Michèle Aké;N’cho Christoph Amin

作者机构：Laboratoire de Chimie Analytique et d’Analyse des Médicaments UFR Pharmacie Faculté des Sciences de la Santé Université d’Abomey-Calavi Cotonou Bénin UFR Pharmacie Faculté des Sciences de la Santé Université d’Abomey-Calavi Cotonou Bénin Département de Chimie Analytique Bromatologie Chimie Générale et Minérale UFR des Sciences Pharmaceutiques et Biologiques Université Félix Houphouë t-Boigny Abidjan Cô te d’Ivoire 

出 版 物：《American Journal of Analytical Chemistry》 (美国分析化学（英文）)

年 卷 期：2022年第13卷第8期

页      面：277-288页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Tartrazine Sunset Yellow HPLC-UV Food Dyes 

摘      要：The use of dyes such as tartrazine (E102) and sunset yellow (E102) in food, beverages and health products for technological and commercial purposes is common. The adverse effects caused by these dyes, such as allergies and hyperactivity disorder have been reported, especially in children. In the present study, a chromatographic method was developed and validated for simultaneous determination of tartrazine and sunset yellow. The chromatographic separation was performed on a Lichrocart® C18 column (125 × 4.6 mm;5 μm) with a security Guard-C18 column (4 × 2.0 mm, 5 μm;Phenomenex, Torrance, CA, USA) maintained at 30°C. The mobile phase consisted of a mixture of acetonitrile/ammonium acetate buffer pH 6.8 in gradient mode with a flow rate of 1 mL/min. The injection volume was 10 μL. The detection wavelength was set at 455 nm. The parameters of specificity, linearity, precision, repeatability, accuracy and sensitivity were examined for validation. The developed method is linear in the range of 1 μg/mL to 100 μg/mL with a R2 0.998. The intra-day and inter-day precisions (RSD) were less than 0.6% and 3.1% respectively. The detection limit was 0.03 μg/mL and the quantification limit was 0.1 μg/mL. The retention time of tartrazine was 2.86 min, while sunset yellow was detected at 5.67 min. A simple, rapid, accurate and robust HPLC/UV-Visible method was developed and validated for simultaneous identification and quantification of tartrazine and sunset yellow. This developed method was successfully applied for the simultaneous determination of tartrazine and sunset yellow in soft drinks sold in Benin.