Quadrupole-Linear Ion Trap Tandem Mass Spectrometry System for Clinical Biomarker Analysis

作     者：Xiang Fang Jie Xie Shiying Chu You Jiang Yuting An Chang Li Xiaoyun Gong Rui Zhai Zejian Huang Chunling Qiu Xinhua Dai Xiang Fang;Jie Xie;Shiying Chu;You Jiang;Yuting An;Chang Li;Xiaoyun Gong;Rui Zhai;Zejian Huang;Chunling Qiu;Xinhua Dai

作者机构：Technology Innovation Center of Mass Spectrometry for State Market RegulationCenter for Advanced Measurement ScienceNational Institute of MetrologyBeijing 100029China College of Instrument Science and Electrical EngineeringJilin UniversityChangchun 130026China 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2022年第16卷第9期

页      面：56-64页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 1010[医学-医学技术（可授医学、理学学位）] 070302[理学-分析化学] 0703[理学-化学] 10[医学] 

基　　金：financially supported by the National Key R&D Program of China(2018YFF0212503,2019YFF0216303,and 2016YFF0200502) the National Natural Science Foundation of China(21927812) the Research Project of the National Institute of Metrology(AKY1934) 

主　　题：Linear ion trap Quadrupole Space charge effect Collision-induced dissociation Biomarker 

摘      要：The accurate and efficient measurement of small molecule disease markers for clinical diagnosis is of great *** this study,a quadrupole-linear ion trap(Q-LIT)tandem mass spectrometer was designed and built in our *** precursor ions were first selected in the quadrupole,and then injected,trapped,and fragmented simultaneously in the linear ion trap(LIT)to reduce the space charge effect,enrich the target product ions,and promote *** targeted analytes were measured with selected reaction monitoring using a positive scan mode with electrospray ionization(ESI).Ions with a mass-to-charge ratio(m/z)ranging from 195 to 2022 were *** scanning at 1218amu.s^(-1),unit resolution and an accuracy of higher than m/z 0.28 was obtained for m/z up to *** dimensionless Mathieu parameter(q)value used in this study was 0.40 for collision-induced dissociation(CID),which was activated by resonance *** an overall CID efficiency of 64%was achieved(activation time,50 ms).Guanidinoacetic acid(GAA)and creatine(CRE)were used as model compounds for small molecule clinical *** limits of quantification were 1.0 and 0.2 nmol.L^(-1)for GAA and CRE,respectively.A total of 77 actual samples were successfully analyzed by the home-built ESI-Q-LIT tandem mass spectrometry *** developed method can reduce matrix interference,minimize space charge effects,and avoid the chromatographic separation of complex samples to simplify the pretreatment *** novel Q-LIT system is expected to be a good candidate for the determination of biomarkers in clinical diagnosis and therapeutics.