Cold plasma promotes Sertoli cell proliferation via AMPK–mTOR signaling pathway

作     者：ZHANG Jiao-jiao LI Ya-qi SHI Mei WANG Yu-sha TANG Yao WANG Xian-zhong ZHANG Jiao-jiao;LI Ya-qi;SHI Mei;WANG Yu-sha;TANG Yao;WANG Xian-zhong

作者机构：Chongqing Key Laboratory of Forage and HerbivoreCollege of Veterinary MedicineSouthwest UniversityChongqing 400715P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第9期

页      面：2700-2719页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31902338) the Natural Science Foundation of Chongqing,China(cstc2019jcyjmsxm X0056) the Innovative Project of Chongqing Returned Overseas Person Entrepreneurship and Innovation Plan,China(cx2020057)。 

主　　题：cold plasma Sertoli cell proliferation AMPK mTOR miRNAs 

摘      要：This study investigated cold plasmas for multiple biological applications. Our previous work has found dielectric barrier discharge plasma improves chicken sperm quality. The number of Sertoli cells(SCs) decides spermatogenesis. However, whether cold plasma can regulate SC proliferation remains unclear. This study explored the effects of cold plasma on immature chicken SC proliferation and the regulation mechanism. Results showed that cold plasma exposure at 2.4 W for 30 s twice with an interval of 6 h produced(P0.05) the maximum SC viability, cell growth, and cell cycle progression. SC proliferation-promoting effect of cold plasma treatment was regulated by increasing(P0.05) the adenosine triphosphate production and the respiratory enzyme activity in the mitochondria. This process was potentially mediated by the adenosine monophosphate-activated protein kinase(AMPK)–mammalian target of rapamycin(m TOR) signaling pathway, which was regulated by the micro RNA(mi RNA) targeting regulation directly and by the intracellular reactive oxygen species homeostasis indirectly. The cold plasma treatment increased(P0.01) the mi R-7450-5 p expression and led to a decreased(P0.01) AMPKα1 level. On the other hand, mi R-100-5 p expression was reduced(P0.05) and led to an increased(P0.05) m TOR level in SCs. A single-stranded synthetic mi R-7450-5 p antagomir and a double-stranded synthetic mi R-100-5 p agomir reduced(P0.05) the SC proliferation. However, this could be ameliorated(P0.05) by the cold plasma treatment. Our findings suggest that appropriate cold plasma treatment provides a safe strategy to improve SC proliferation, which is beneficial to elevating male chicken reproductive capacity.