Quantitative analysis of collagen morphology in breast cancer from millimeter scale using multiphoton microscopy

作     者：Yulan Liu Shunwu Xu Deyong Kang Xingxin Huang Shuoyu Xu Lianhuang Li Liqin Zheng Lida Qiu Zhenlin Zhan Xiahui Han Jianxin Chen 

作者机构：Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal UniversityFuzhou 350007P.R.China School of Electronic and Mechanical Engineering Fujian Polytechnic Normal University FuqingFujian 350300P.R.China Department of Pathology Fujian Medical University Union HospitalFuzhou 350001 P.R.China Department of General SurgeryNanfang Hospital Southern Medical University Guangzhou 510515P.R.China College of Physics and Electronic Information Engineering Minjiang UniversityFuzhou 350108P.R.China 

出 版 物：《创新光学健康科学杂志(英文)》 (Journal of Innovative Optical Health Sciences)

年 卷 期：2023年第16卷第4期

页      面：48-57页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0808[工学-电气工程] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0836[工学-生物工程] 0702[理学-物理学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (Grant Nos.82171991 and 81700576) Natural Science Foundation of Fujian Province (Nos.2020J01154,2020J011008,2019J01269 and 2020J01839) Joint Funds for the Innovation of Science and Technology of Fujian Province (2017Y9038 and 2019Y9101) the special Funds of the Central Government Guiding Local Science and Technology Development (No.2020L3008) 

主　　题：Breast cancer tumor microenvironment collagen¯ber morphology multiphoton microscopy 

摘      要：The tumor microenvironment(TME)is now recognized as an important participant of tumor *** the most abundant extracellular matrix component in TME,collagen plays an important role in tumor *** imaging study of collagen morphological feature in TME is of great significance for understanding the state of *** microscopy(MPM),based on second harmonic generation(SHG)and two-photon excitation fluorescence(TPEF),can be used to monitor the morphological changes of biological tissues without *** this study,we used MPM for large-scale imaging of early invasive breast cancer from the tumor center to normal tissues far from the *** found that there were signi¯cant di®erences in collagen morphology between breast cancer tumor boundary,near tumor transition region and normal tissues far from the ***,the morphological feature of eight collagen¯bers was extracted to quantify the variation trend of collagen in three *** results may provide a new perspective for the optimal negative margin width of breast-conserving surgery and the understanding of tumor metastasis.