The protein-protein interaction landscape of transcription factors during gynoecium development in Arabidopsis
作者机构：Unidad de Genómica Avanzada(UGA-LANGEBIO)Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional(CINVESTAV-IPN)IrapuatoGuanajuato 36824México Departamento de Biotecnología y BioquímicaUnidad IrapuatoCINVESTAV-IPNIrapuatoGuanajuato 36824México Instituto de FísicaUniversidad de San Luis PotosíSan Luis PotosíSLP 78290México Present address:Department of Plant SciencesUniversity of CambridgeCambridgeUK Present address:Department of Molecular and Cellular BiologyUniversity of California DavisDavisCAUSA Present address:CONACYTInstituto de FitosanidadColegio de PostgraduadosCampus MontecilloTexcocoMéxico
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2023年第16卷第1期
页 面：260-278页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：H.H.-U.,V.L.-G.,and P.L.-G.were supported by the Mexican National Council of Science and Technology(CONACyT)with a Ph.D.fellowship(243380,487657,and 747095,respectively) Work in the S.d.F.laboratory was financed by the CONACYT grants CB-2012-177739,FC-2015-2/1061,and CB-2017-2018-A1-S-10126,and N.M.-M.by the CONACYT grant CB-2015-255069.S.d.F.is grateful for the Fellowship of the Marcos Moshinsky Foundation(2018) participation in the European Union projects H2020-MSCA-RISE-2020 EVOfruland project(101007738)and H2020-MSCA-RISE-2019 MAD project(872417).
主 题：interactome networks transcription factors gynoecium development
摘 要：Flowers are composed of organs whose identity is defined by the combinatorial activity of transcription factors(TFs).The interactions between MADS-box TFs and protein complex formation have been schematized in the floral quartet model of flower development.The gynoecium is the flower’s female reproductive part,crucial for fruit and seed production and,hence,for reproductive success.After the establishment of carpel identity,many tissues arise to form a mature gynoecium.TFs have been described as regulators of gynoecium development,and some interactions and complexes have been identified.However,broad knowledge about the interactions among these TFs and their participation during development remains scarce.In this study,we used a systems biology approach to understand the formation of a complex reproductive unit—as the gynoecium—by mapping binary interactions between well-characterized TFs.We analyzed almost 4500 combinations and detected more than 250 protein-protein interactions(PPIs),resulting in a process-specific interaction map.Topological analyses suggest hidden functions and novel roles for many TFs.In addition,we observed a close relationship between TFs involved in auxin and cytokinin-signaling pathways and other TFs.Furthermore,we analyzed the network by combining PPI data,expression,and genetic data,which helped us to dissect it into several dynamic spatio-temporal subnetworks related to gynoecium development processes.Finally,we generated an extended PPI network that predicts new players in gynoecium development.Taken together,all these results serve as a valuable resource for the plant community.
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