Single-Atomic Site Catalyst Enhanced Lateral Flow Immunoassay for Point-of-Care Detection of Herbicide

作     者：Zhaoyuan Lyu Shichao Ding Peter Tieu Lingzhe Fang Xin Li Tao Li Xiaoqing Pan Mark H.Engelhard Xiaofan Ruan Dan Du Suiqiong Li Yuehe Lin 

作者机构：School of Mechanical and Material EngineeringWashington State UniversityPullmanWA 99164USA Department of ChemistryUniversity of CalforniaIrvineIrvineCA 92697USA Department of Chemistry and BiochemistryNorthern Ilinois UniversityDeKalbIL 60115USA X-ray Science DivisionArgonne National LaboratoryLemontIL 60439USA Irvine Materials Research Institute(IMRI)Department of Materials Science and EngineeringUniversity of CaliforniaIrvineIrvineCA92697USA Environmental Molecular Sciences LaboratoryPacific Northwest National LaboratoryRichlandWA 99354USA DL ADV-TechPullmanWA 99163USA 

出 版 物：《Research》 (研究（英文）)

年 卷 期：2023年第2022卷第1期

页      面：63-74页

核心收录：

学科分类：081705[工学-工业催化] 08[工学] 0817[工学-化学工程与技术] 

基　　金：support from the National Institute of Environmental Health Sciences of the US National Institutes of Health(Award Number 1R43ES031885-01) 

主　　题：catalyst replace dispersed 

摘      要：Point-of-care(POC)detection of herbicides is of great importance due to their impact on the environment and potential risks to human ***,we design a single-atomic site catalyst(SASC)with excellent peroxidase-like(POD-like)catalytic activity,which enhances the detection performance of corresponding lateral flow immunoassay(LFIA).The iron single-atomic site catalyst(Fe-SASC)is synthesized from hemin-doped ZIF-8,creating active sites that mimic the Fe active center coordination environment of natural enzyme and their *** to its atomically dispersed iron active sites that result in maximum utilization of active metal atoms,the Fe-SASC exhibits superior POD-like activity,which has great potential to replace its natural ***,the catalytic mechanism of Fe-SASC is systematically *** its outstanding catalytic activity,the Fe-SASC is used as label to construct LFIA(Fe-SASC-LFIA)for herbicide *** 2,4-dichlorophenoxyacetic acid(2,4-D)is selected as a target here,since it is a commonly used herbicide as well as a biomarker for herbicide exposure evaluation.A linear detection range of 1-250 ng/mL with a low limit of detection(LOD)of 0.82 ng/mL has been ***,excellent specificity and selectivity towards 2,4-D have been *** outstanding detection performance of the Fe-SASC-LFIA has also been demonstrated in the detection of human urine samples,indicating the practicability of this POC detection platform for analyzing the 2,4-D exposure level of a *** believe this proposed Fe-SASC-LFIA has potential as a portable,rapid,and high-sensitive POC detection strategy for pesticide exposure evaluation.