Efficacy of green tea extract on PC3 prostate cancer cells through upregulation of miR-195 expression and suppression of epithelial to mesenchymal transition

作     者：Fatemeh Safari Katayoun Dadeh Amirfard Fatemeh Safari;Katayoun Dadeh Amirfard

作者机构：Department of BiologyFaculty of ScienceUniversity of GuilanRasht ***Iran Department of MicrobiologyNorth Tehran BranchIslamic Azad UniversityTehran ***Iran 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第5期

页      面：681-686页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Green tea extract PC3 cancer cells epithelial-mesenchymal transition miR-195 expression 

摘      要：OBJECTIVE: To evaluate anticancer efficacy of green tea extract(GTE) on PC3 prostate cancer cells. METHODS: By using quantitative real-time polymerase chain reaction(qRT-PCR) and Western blot methods, the expression of miR-195 and the epithelial to mesenchymal transition(EMT) markers such as E-cadherin and vimentin was analyzed. RESULTS: Based on the results of 2D and 3D cell culture models, the inhibition of EMT and up regulation of miR-195 expression were detected. CONCLUSIONS: Our findings will be helpful to design anti-tumor regimens with natural product original, and more studies will be required to identify the related mechanisms involving anticancer activities of green tea via miRNAs.