Comparison of pretreatment,preservation and determination methods for foliar pH of plant samples

作     者：Sining Liu Jiashu Chen Wenxuan Han 

作者机构：Department of Ecology and Ecological EngineeringBeijing Key Laboratory of Biodiversity and Organic FarmingCollege of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan ProvinceInstitute of Ecology&ForestryCollege of ForestrySichuan Agricultural UniversityChengdu 611130China 

出 版 物：《Journal of Plant Ecology》 (植物生态学报（英文版）)

年 卷 期：2022年第15卷第4期

页      面：673-682页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the‘Strategic Priority Research Program’of the Chinese Academy of Sciences(XDA26040202) the National Natural Science Foundation of China(32001165) supported by Chinese Universities Scientifc Fund(2021TC117) 

主　　题：green/senesced leaf leaf pH plant functional traits sample preservation specifcation/standard/protocol leaf water ratio transformation/conversion equation 

摘      要：To compare current methods of pretreatment/determination for plant foliar pH,we proposed a method for longperiod sample preservation with little interference with the stability of foliar *** hundred leaf samples from 20 species were collected and four methods of pH determination were used:refrigerated(stored at 4°C for 4 days),frozen(stored at−16°C for 4 days),oven-dried and fresh green-leaf pH(control).To explore the effects of different leaf:water mixing ratio on the pH determination results,we measured oven-dried green-leaf pH by leaf:water volume ratio of 1:8 and mass ratio of 1:10,and measured frozen senesced-leaf pH by mass ratio of 1:10 and 1:*** standard major axis regression was used to analyze the relationship and the conversion equation between the measured pH with different *** pH of refrigerated and frozen green leaves did not signifcantly differ from that of fresh green-leaf,but drying always overrated fresh green-leaf *** the feld sampling,cryopreservation with a portable refrigerator was an advisable choice to get a precise *** long-duration feld sampling,freezing was the optimal choice,and refrigeration is the best choice for the shorttime *** different leaf:water mixing ratio signifcantly infuenced the measured foliar *** dilution reduced the proton concentration and increased the measured *** fndings provide the conversion relationships between the existing pretreatment and measurement methods,and establish a connection among pH determined by different *** study can facilitate foliar pH measurement,thus contributing to understanding of this interesting plant functional trait.