Dissection the endocytic routes of viral capsid proteins-coated upconversion nanoparticles by single-particle tracking

作     者：Yujun Ning Lin Wei Shen Lin Yifan Jiang Naidong Wang Lehui Xiao Yujun Ning;Lin Wei;Shen Lin;Yifan Jiang;Naidong Wang;Lehui Xiao

作者机构：College of ChemistryZhengzhou UniversityZhengzhou 450001China State Key Laboratory of Medicinal Chemical BiologyTianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071China College of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410081China College of Veterinary MedicineHunan Agricultural UniversityChangsha 410128China School of Chemistry and Chemical EngineeringHenan Normal UniversityXinxiang 453007China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第10期

页      面：4710-4714页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 07[理学] 070205[理学-凝聚态物理] 0703[理学-化学] 0702[理学-物理学] 10[医学] 

基　　金：financial support from the National Natural Science Foundation of China(Nos.22174079 21974073). 

主　　题：Upconversion nanoparticles Viral capsid proteins Single-particle tracking Hepatoma cells Endocytosis 

摘      要：Real-time exploring the cellular endocytic pathway of viral capsid proteins(VCPs)functionalized nanocargos at the single-particle level can provide deep insight into the kinetic information involved in virus infection.In this work,porcine circovirus type 2(PCV2)VCPs with different functions are modified onto the surface of upconversion nanoparticles(VCPs-UCNPs)to investigate the cellular internalization process in real-time.Clathrin-mediated endocytosis is found to be the essential uptake mechanism for these VCPs-UCNPs.Besides,it is verified that P_(1)-UCNPs(PCV2 VCPs with nuclear localization signal,namely P1)can be easily assembled close to the perinuclear area,which is different from that of P_(2)-UCNPs(PCV2 VCPs without nuclear localization signal,namely P_(2)).Interestingly,multistep entry processes are observed.Particularly,confined diffusion is observed during the transmembrane process.The intracellular transport of VCPs-UCNPs is dependent on microtubules toward the cell interior.During this process,P_(1)-UCNPs display increased velocities with active transport,while diffusion much faster around the perinuclear area.But for P_(2)-UCNPs,there are only two phases involved in their endocytosis process.This study presents distinct dynamic mechanisms for the nanocargos with different functions,which would make a useful contribution to the development of robust drug delivery systems.