Bifidobacterium infantis regulates the programmed cell death 1 pathway and immune response in mice with inflammatory bowel disease

作     者：Lin-Yan Zhou Ying Xie Yan Li 

作者机构：Department of GastroenterologyShengjing Hospital of China Medical UniversityShenyang 110004Liaoning ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2022年第28卷第26期

页      面：3164-3176页

核心收录：

学科分类：1002[医学-临床医学] 100202[医学-儿科学] 10[医学] 

基　　金：Supported by the Doctoral Start-up Foundation of Liaoning Province No.2021-BS-114 

主　　题：Bifidobacterium infantis Enteritis Programmed cell death ligand T-Lymphocytes 

摘      要：BACKGROUND Inflammatory bowel disease(IBD)is caused by an abnormal immune *** cell death 1(PD-1)is an immunostimulatory molecule,which interacts with PD ligand(PD-L1)playing a prime important role among autoimmune *** infantis(***)can promote the differentiation of CD(cluster of differentiation)4^(+)T cells into regulatory T cells(Tregs).Tregs participate in the development of IBD and may be related to disease *** amplify the expression level of PD-1,PD-L1 and Tregs’nuclear transcription factor forkhead box protein 3(Foxp3).But the mechanism of *** on PD-1/PD-L1 signaling remains *** To explore the mechanism of *** regulating the immune response in *** Forty-eight-week-old BALB/c mice were randomly divided into five groups:The control group,dextran sulphate sodium(DSS)model group,DSS+*** group,DSS+***+anti-PD-L1 group,and DSS+anti-PD-L1 *** control group mice were given drinking water freely,the other four groups were given drinking water containing 5%DSS *** control group,DSS model group,and DSS+anti-PD-L1 group were given normal saline(NS)400μL daily by gastric lavage,and the DSS+*** group and DSS+***+anti-PDL1 group were given NS and 1×109 colony-forming unit of *** daily by gastric *** DSS+***+anti-PD-L1 group and DSS+anti-PD-L1 group were given 200μg of PD-L1 blocker intraperitoneally at days 0,3,5,and 7;the control group,DSS+anti-PD-L1 group,and DSS+*** group were given an intraperitoneal injection of an equal volume of phosphate buffered saline(PBS).Changes in PD-L1,PD-1,Foxp3,interleukin(IL)-10,and transforming growth factorβ(TGF-β)1 protein and gene expression were *** cytometry was used to observe changes in CD4^(+),CD25^(+),Foxp3^(+)cell numbers in the blood and *** Compared to the control group,the expression of PD-1,Foxp3,IL-10,and TGF-β1 was significantly decreased in the int