Dithizone-functionalized C18 online solid-phase extraction-HPLC-ICP-MS for speciation of ultra-trace organic and inorganic mercury in cereals and environmental samples

作     者：Ying Wang Ailing Zhu Yingying Fang Changjun Fan Yanli Guo Zhiqiang Tan Yongguang Yin Yong Cai Guibin Jiang Ying Wang;Ailing Zhu;Yingying Fang;Changjun Fan;Yanli Guo;Zhiqiang Tan;Yongguang Yin;Yong Cai;Guibin Jiang

作者机构：Laboratory of Environmental Nanotechnology and Health EffectResearch Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China State Key Laboratory of Environmental Chemistry and EcotoxicologyResearch Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of SciencesBeijing 100049China School of EnvironmentHangzhou Institute for Advanced StudyUCASHangzhou 310024China Shimadzu China Innovation CenterShimadzu(China)Co.LTDBeijing 100020China Department of Chemistry and BiochemistryFlorida International UniversityMiamiFL 33199United States 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2022年第34卷第5期

页      面：403-410页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 0804[工学-仪器科学与技术] 

基　　金：This work was supported by the National Key Research and Development Project(No.2020YFA0907400) the National Natural Science Foundation of China(Nos.21777178,21976193) Y.Yin acknowledges the supports from the National Young Top-Notch Talents(No.W03070030) the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.Y202011). 

主　　题：Mercury speciation Online solid-phase extraction HPLC-ICP-MS Dithizone Cereals Natural water 

摘      要：A simple and efficient dithizone-functionalized solid-phase extraction(SPE)procedure,online coupled with high-performance liquid chromatography(HPLC)-inductively coupled plasma mass spectrometry,was developed for the first time for enrichment and determination of ultra-trace mercury(Hg)species(inorganic divalent Hg(Hg(Ⅱ)),methylmercury(CH_(3)Hg(Ⅱ))and ethylmercury(C_(2)H_(5)Hg(Ⅱ))in cereals and environmental samples.In the proposed method,functionalization of the commercial C_(18) column with dithizone,enrichment,and elution of the above Hg species can be completed online with the developed SPE device.A simple solution of 2-mercaptoethanol(1%(V/V))could be used as an eluent for both the SPE and HPLC separation of Hg species,significantly simplifying the method and instrumen-tation.The online SPE method was optimized by varying dithizone dose,2-mercaptoethanol concentration,and sample volume.In addition,the effect of pH,coexisting interfering ions,and salt effect on the enrichment was also discussed.Under the optimized conditions,the detection limits of Hg species for 5 mL water sample were 0.15 ng/L for Hg(Ⅱ),0.07 ng/L for CH_(3)Hg(Ⅱ),and 0.04 ng/L for C_(2)H_(5)Hg(Ⅱ)with recoveries in the range of 85%-100%.The developed dithizone-functionalized C_(18) SPE column can be reused after a single function-alization,which significantly simplifies the enrichment step.Moreover,the stability of Hg species enriched on the SPE column demonstrated its suitability for field sampling of Hg species for later laboratory analysis.This environment-friendly method offers a robust tool to detect ultra-trace Hg species in cereals and environmental samples.