Selection and Verification of Reference Genes for qRT-PCR Analysis in Iris domestica under Drought
作者机构：College of Chinese Medicinal MaterialsJilin Agricultural UniversityChangchun130118China
出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)
年 卷 期：2022年第91卷第11期
页 面：2537-2548页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：funded by the Modern Agricultural Industrial Technology System Project(CARS-21)
主 题：Iris domestica gene expression qRT-PCR reference genes drought
摘 要：Iris domestica is a plant of the Iridaceae family and is drought-tolerant,but its drought-resistance mechanism is not yet *** the gene expression changes of *** by qRT-PCR is an important mean to understand its drought resistance ***,a lack of reference genes greatly hinders investigation and research on the adaptation of *** to drought at the molecular and genetic *** this study,we assessed the expression stability of 11 candidate gene in *** under drought stress conditions and different tissues using geNorm,NormFinder,BestKeeper and RefFinder *** results showed that EF1βwas the most stable reference genes under drought stress and in different *** validate further the stability of the identified reference genes,the expression patterns of VP gene in *** was *** results will be conducive to more accurate quantification of gene expression levels in ***.
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