Protective effects and mechanisms of Lizhong decoction(理中汤) against non-alcoholic fatty liver disease in a rat model

作     者：YANG Jiayao TAO Dongqing MA Wei LIU Song LIAO Yan SHU Lei ZHANG Shu LI Chenyu DU Nianlong YANG Jiayao;TAO Dongqing;MA Wei;LIU Song;LIAO Yan;SHU Lei;ZHANG Shu;LI Chenyu;DU Nianlong

作者机构：Department of GastroenterologyWuhan Integrated TCM and Western Medicine HospitalWuhan 430022China Department of EndocrinologyThe Third People's Hospital of Hubei ProvinceWuhan 430033China Department of Center LaboratoryWuhan Integrated TCM and Western Medicine HospitalWuhan 430022China Hubei University of Traditional Chinese MedicineWuhan 430065China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第5期

页      面：773-780页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Backbone Talents for TCM Clinical Characteristic Technology Inheritance Foundation of State Administration of Traditional Chinese Medicine:Protective Effects and Mechanisms of Lizhong Decoction Against Non-Alcoholic Fatty Liver Disease in a Rat Model [National Education Letter of Traditional Chinese Medicine (2018) No. 224] 

主　　题：non-alcoholic fatty liver disease PPAR gamma dipeptidyl peptidase 4 Lizhong decoction 

摘      要：OBJECTIVE: To investigate the protective effects and molecular mechanisms of Lizhong decoction( 理中汤, LZD) against non-alcoholic fatty liver disease(NAFLD). METHODS: Male Wistar rats were fed a high-fat diet for four weeks to induce NAFLD, and were administered LZD by gavage for four weeks. Potential therapeutic targets for NAFLD were analyzed using network pharmacology. Liver pathology was evaluated using Oil Red O and hematoxylin-eosin staining. Furthermore, mitochondrial function, lipid metabolism, oxidative stress, and inflammatory response were examined. RESULTS: Rats with NAFLD exhibited high levels of hepatic damage and cholesterol deposition. Moreover, apoptosis was increased, superoxide dismutase and glutathione content were reduced, malondialdehyde content was increased, and the protein expression of inflammatory cytokines and p-c-Jun N-terminal kinase was increased. The LZD treatment ameliorated mitochondrial dysfunction, reduced liver damage,inhibited oxidative stress and inflammatory response, upregulated peroxisome proliferator-activated receptor(PPAR)-γ expression, and suppressed dipeptidyl peptidase 4(DPP4) expression in the liver. CONCLUSION: It was found that LZD alleviates NAFLD by activating PPAR-γ and inhibiting DPP4.