Paper-based fluorescent devices for multifunctional assays:Biomarkers detection,inhibitors screening and chiral recognition

作     者：Wang Li Xiaoyue Zhang Siqi Chen Yibing Ji Ruijun Li Wang Li;Xiaoyue Zhang;Siqi Chen;Yibing Ji;Ruijun Li

作者机构：Department of Analytical ChemistryChina Pharmaceutical UniversityNanjing 210009China Key Laboratory of Drug Quality Control and PharmacovigilanceMinistry of EducationNanjing 210009China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第9期

页      面：4405-4410页

核心收录：

学科分类：07[理学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：financially supported by the National Natural Science Foundation of China (No. 21804141) “Double First-Class University” Project (Nos. CPU2018GY07 and CPU2018GY21) 

主　　题：Paper-based devices Multifunctional Xanthine Inhibitor screening Chiral recognition 

摘      要：The development of a single analytical platform with different functions is highly desirable but remains a challenge at present.Here,a paper-based device based on fluorescent carbon dots(CDs)functionalized paper/MnO_(2)nanosheets(MnO_(2)NS)hybrid devices(PCD/NS)was proposed for single-device multi-function applications.MnO_(2)NS functioned as a fluorescence quencher of CDs and recognizer of H_(2)O_(2)released from the oxidase catalyzed system.Fluorescence recovery would occur after the decomposition of MnO_(2)NS induced by H_(2)O_(2),by which a simple and effective strategy could be developed for fluorescence monitoring multiplex biological events.Xanthine(XA)sensing,xanthine oxidase(XOD)inhibitors screening analysis and chiral recognition of glucose enantiomers were performed on PCD/NS to investigate the multifunctional application of the paper-based device.By means of PCD/NS,XA could be determined in the range of 0.1–40μmol/L with a low detection of limit of 0.06μmol/L.The IC_(50)value of allopurinol,the model inhibitor of XOD,was sensitively detected to be 7.4μmol/L.Glucose enantiomers were also recognized in terms of the specific fluorescence response to d-glucose.This work firstly presented a paper-based device capable of biomarkers detection,inhibitors screening and chiral recognition,which enlightened a promising strategy for the construction of multifunctional devices and hold the great potential application in clinical diagnosis and drug discovery.