CD14 macrophage and IL-10 levels in the peripheral blood of breast cancer patients and their diagnostic value

作     者：Mengting Dong Jiayu Sheng Chunyang Li Patiguli·JIAPAER Xiaofei Li Minjia Yuan Xiaohong Xue Ke Jiang Mengting Dong;Jiayu Sheng;Chunyang Li;Patiguli·JIAPAER;Xiaofei Li;Minjia Yuan;Xiaohong Xue;Ke Jiang

作者机构：Department of Breast DiseasesYueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 200437China 

出 版 物：《Oncology and Translational Medicine》 (肿瘤学与转化医学（英文版）)

年 卷 期：2022年第8卷第4期

页      面：165-172页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by grants from the National Natural Science Foundation of China(No.82104952,No.82004240) Special project for clinical research of health industry of Shanghai Municipal Health Commission(No.202140172) Special project of medical innovation research of Shanghai Science and Technology Commission(No.21Y11923600) the Shanghai Office of Traditional Chinese Medicine Development(No.ZY2018-2020-RCPY-2009) 

主　　题：breast cancer macrophage IL-10 peripheral blood diagnostic value 

摘      要：Objective To explore the correlation between macrophages and interleukin-10(IL-10 in the peripheral blood of breast cancer(BC)patients and the diagnostic value of joint *** BC patients(n=50)and healthy controls(n=40)were prospectively *** percentage of circulating cluster of differentiation 14(CD 14)macrophage cells was analyzed by flow cytometry,and an enzyme-linked immunosorbent assay(ELISA)was used to detect IL-10 expression *** operating characteristic(ROC)curves were used to verify the diagnostic value of the models based on the expression of CD14 macrophage cell populations and *** addition,the association between model expression and clinicopathological characteristics was *** 30 patients with BC and 30 with benign breast disease were selected to validate the IL-10 and CD14 macrophage joint detection model using the same *** CD14 macrophage and IL-10 expression levels in BC patients were higher than those in healthy controls(P0.05).The ROC curve showed that the area under the curve(AUC)of CD14+macrophages combined with IL-10 was 0.830,the sensitivity was 72.0%,and the specificity was 87.5%.Its diagnostic efficiency was better than all other single and joint *** analysis of clinicopathological features showed that IL-10 and CD14+macrophage joint detection was significantly correlated with tumor size,tumor-node-metastasis(TNM)stage,and lymph node,estrogen receptor(ER),and Ki-67 expression(P0.05).The validation analysis results were consistent with the test *** Peripheral blood macrophages can be an independent diagnostic marker for *** detection of CD14-macrophages and IL-10 suggests poor prognosis,which has unlimited potential to guide BC development and provides a new theory for studying tumor-associated macrophages in BC.