Supercontinuum fiber laser-based coherent anti-Stokes Raman scattering microscopy for label-free chemical imaging
作者机构：Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information School of Life Science and Technology Xidian UniversityXi'anShaanxi 710126P.R.China Engineering Research Center of Molecular&Neuro Imaging of the Ministry of Education Xidian UniversityXi'anShaanxi 710126P.R.China Shaanxi Eye Hospital Xi'an People's Hospital(Xi'an Fourth Hospital)Affiliated Guangren HospitalSchool of Medicine Xi'an Jiaotong UniversityXi'an 710004P.R.China
出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)
年 卷 期：2022年第15卷第4期
页 面：108-115页
核心收录：
学科分类：080901[工学-物理电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0803[工学-光学工程]
基 金：This work was supported in part by the National Key R&D Program of China(2018YFC0910600) the National Natural Science Foundation of China(81871397) the National Young Talent Program,the Shaanxi Science Fund for Distinguished Young Scholars(2020JC-27) the Key Research and Development Program of Shaanxi(2021ZDLSF04-05) the Shaanxi Young Top-notch Talent Program,the Best Funded Projects for the Scientific and Technological Activities for Excellent Overseas Researchers in Shaanxi Province(2017017) the Fundamental Research Funds for Central Universities(QTZX2105) Xueli Chen would like to thank Dr.Chi Zhang at Purdue University for his help in building the CARS microscope
主 题：Coherent anti-Stokes Raman scattering supercontinuum fiber laser lipid mapping cancer cell
摘 要：Coherent anti-Stokes Raman scattering(CA RS)microscopy can resolve the chemical compo-nents and distribution of living biological systems in a label-firee manner and is favored in several *** CA RS microscopes typically use bulky,high-performance solid-state lasers,which are expensive and sensitive to environmental *** their relatively low cost and environmental sensitivity,supercontinum fiber(SF)lasers with a small footprint have found increasing use in biomedical *** these features,in this paper,we homebuilt a low-cost CARS microscope based on a SF laser module(scCA RS microscope).This SF laser module is specially customized by adding a time synchronized seed source channel to the SF laser to form a dual-channel output *** performance of the scCARS microscope is evaluated with dimethyl sulfoxide,whose results confirm a spatial resolution of better than 500nm and a detection sensitivity of millimolar *** dual-color imaging capability is further demonstrated by imaging different species of mixed *** finally explore the potential of our scCARS microscope by mapping lipid droplets in different cancer cells and corneal stromal lenses.
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