Hepatocellular carcinoma-derived exosomal miRNA-761 regulates the tumor microenvironment by targeting the SOCS2/JAK2/STAT3 pathway

作     者：Xiao-hu Zhou Hao Xu Chang Xu Ying-cai Yan Lin-shi Zhang Qiang Sun Wei-lin Wang Yan-jun Shi Xiao-hu Zhou;Hao Xu;Chang Xu;Ying-cai Yan;Lin-shi Zhang;Qiang Sun;Wei-lin Wang;Yan-jun Shi

作者机构：Department of Hepatobiliary and Pancreatic Surgerythe Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Provincethe Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China 

出 版 物：《World Journal of Emergency Medicine》 (世界急诊医学杂志（英文）)

年 卷 期：2022年第13卷第5期

页      面：379-385页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China(82072203) Natural Science Foundation of Zhejiang Province(LQ19H160025) 

主　　题：Exosomes Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)signaling pathway microRNA-761 Suppressor of cytokine signaling 2 Tumor microenvironment 

摘      要：BACKGROUND:Exosomes and exosomal microRNAs have been implicated in tumor occurrence and *** previous study showed that microRNA-761(miR-761)is overexpressed in hepatocellular carcinoma(HCC)tissues and that its inhibition affects mitochondrial function and inhibits HCC *** mechanism by which exosomal miR-761 modulates the tumor microenvironment has not been ***:Exosomal miR-761 was detected in six cell *** counting kit-8(CCK-8)and transwell migration assays were performed to determine the function of exosomal miR-761 in HCC *** luciferase reporter assay was used to analyze miR-761 target genes in normal fi broblasts(NFs).The inhibitors AZD1480 and C188-9 were employed to determine the role of the Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)signaling pathway in the transformation of cancer-associated fi broblasts(CAFs).RESULTS:In this study,we characterized the mechanism by which miR-761 reprogrammed the tumor *** found that HCC-derived exosomal miR-761 was taken up by ***,HCC exosomes aff ected the tumor microenvironment by activating NFs via suppressor of cytokine signaling 2(SOCS2)and the JAK2/STAT3 signaling ***:These results demonstrated that exosomal miR-761 modulated the tumor microenvironment via SOCS2/JAK2/STAT3 pathway-dependent activation of *** fi ndings may inspire new strategies for HCC prevention and therapy.