Celastrol inhibits laser-induced choroidal neovascularization by decreasing VEGF induced proliferation and migration

作     者：Zhen Li Ke-Wen Zhou Fang Chen Fu Shang Ming-Xing Wu 

作者机构：State Key Laboratory of OphthalmologyZhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina Department of PhysiologyZhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080Guangdong ProvinceChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2022年第15卷第8期

页      面：1221-1230页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China(No.81570826) 

主　　题：celastrol choroidal neovascularization proliferation vascular endothelial growth factor human choroidal endothelial cells 

摘      要：AIM:To evaluate celastrol s effect on choroidal neovascularization(CNV).METHODS:In this study,neovascular formation in vitro(tube formation and aortic ring culture)and in vivo(laser induced neovascular in mice)was treated with celastrol to evaluate this natural compound s impact on *** blot was applied to explore the possible mechanism for *** in vitro assay,triplicate for each group was repeated at least three *** in vivo assay,each group contains 5 ***:Celastrol supressed tube formation and aortic ring sprout *** vitro assay exhibited that celastrol inhibiting vascular endothelial growth factor(VEGF)-induced proliferation and migration of human umbilical vein endothelial cells and human choroidal endothelial cells,and by blocking VEGF ***,intraperitoneal administration of celastrol significantly reduced the area of laser-induced CNV in an in vivo mouse *** day 14,the area of CNV had decreased by 49.15%and 80.26%in the 0.1 mg/kg celastrol-treated group(n=5)and in the 0.5 mg/kg celastrol treated group(n=5),respectively,compared to the vehicle-treated group(n=5).CONCLUSION:Celastrol inhibits CNV by inhibiting VEGF-induced proliferation and migration of vascular endothelial cells,indicating that celastrol is a potent,natural therapeutic compound for the prevention of CNV.