Real-time tracking of ER turnover during ERLAD by a rhenium complex via lifetime imaging
Real-time tracking of ER turnover during ERLAD by a rhenium complex via lifetime imaging作者机构：MOE Key Laboratory of Bioinorganic and Synthetic ChemistrySchool of ChemistryState Key Laboratory of Oncology in South China Sun Yat-Sen University
出 版 物：《National Science Review》 (国家科学评论(英文版))
年 卷 期：2022年第9卷第7期
页 面：80-87页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 0703[理学-化学] 0702[理学-物理学]
基 金：supported by the National Natural Science Foundation of China (22022707, 21778078, 21837006, 91953117 and22177142) Fundamental Research Funds for the Central Universities
主 题：rhenium ER-phagy viscosity TPFLIM
摘 要：Endoplasmic reticulum(ER) degradation by autophagy(ER-phagy) is a recently revealed selective autophagy pathway that plays important roles in organelle turnover and protein degradation,but the biological functions of ER-phagy are largely unknown.Here,we present an ER-targeting Re(I) tricarbonyl complex(Re-ERLAD) that can accumulate in the ER,induce ER-to-lys osome-associated degradation(ERLAD) upon visible light irradiation,and label ER buds and track their morphological alterations during ER-phagy.The emission of Re-ERLAD is sensitive to viscosity,which is a key parameter reflecting the amount of unfolded protein in the ER.Quantitative detection using two-photon fluorescence lifetime imaging microscopy shows that ER viscosity initially increases and then decreases during ERLAD,which reveals that ERLAD is a pathway for alleviating ER stress caused by unfolded proteins.In conclusion,our work presents the first specific photoinducer and tracker of ERLAD,which can be used in studying the regulatory mechanism and function of this process.
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