Determination ofβ-sitosterol in Plumbago zeylanica *** HPLC-ELSD

作     者：Hongyu ZHAO 

作者机构：Market Supervision Service Center of Dandong City Dandong 118001 China 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2022年第13卷第3期

页      面：6-8页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Plumbago zeylanica L. β-sitosterol Content HPLC-ELSD method 

摘      要：[Objectives]A method for the determination ofβ-sitosterol in Plumbago zeylanica *** established and the content ofβ-sitosterol in different medicinal parts,different producing areas and different harvest periods were compared.[Methods]High performance liquid chromatography(HPLC)-evaporative light scattering detector assay was *** chromatographic column was Kromasil C_(18) column(250 mm×4.6 mm,5μm);the mobile phase was pure methanol;the flow rate was 1.0 mL/min;the column temperature was 30℃.The detection parameters of evaporative light scattering detector were as follows:drift tube temperature was 40℃,carrier gas(N_(2))pressure was 3.5 bar.[Results]There was a good linear relationship betweenβ-sitosterol(1.080-4.860μg)and the natural logarithm of peak area(r=0.9995).The average recovery rate was 99.80%.The content ofβ-sitosterol in root and stem was 0.2074 and 0.4064 mg/g,respectively,but it was not found in leaves;the content ofβ-sitosterol in *** *** Guangxi was generally lower than that in Yunnan,and the content ofβ-sitosterol in *** *** Xishuangbanna was the highest;the content ofβ-sitosterol in the stem of *** *** stable at a relatively high level in different harvest periods.[Conclusions]The method is simple,accurate and reproducible,and can be used as one of the methods to control the quality of *** L.