Single-wavelength-excited fluorogenic nanoprobe for accurate realtime ratiometric analysis of broad pH fluctuations in mitophagy
作者机构：Department of PhysicsChemistryand Biology(IFM)Linköping UniversityLinköping SE 58183Sweden School of Chemistry and Chemical EngineeringAnhui UniversityHefei 230601China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2022年第15卷第7期
页 面：6515-6521页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 08[工学] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）]
基 金：supported by STINT Joint China-Sweden Mobility Project(No.CH2017-7243) the Swedish Research Council(VR)(Nos.2019-02409 and 2020-05437) the China Scholarship Council(CSC),Carl Tryggers Stiftelse(No.CTS 19:379) Swedish Government strategic faculty grant in material science(SFO,MATLIU)in Advanced Functional Materials(AFM)(VR No.5.1-2015-5959) the Centre in Nano Science and technology at LiTH(CeNano),and LiU Cancer network at Linköping University.
主 题：mitochondrial pH mitophagy ratiometric fluorescence nanoprobe cell imaging
摘 要：Mitophagy has a critical role in maintaining cellular homeostasis through acidic lysosomes engulfing excess or impaired mitochondria,thereby pH fluctuation is one of the most significant indicators for tracking mitophagy.Then such precise pH tracking demands the fluorogenic probe that has tailored contemporaneous features,including mitochondrial-specificity,excellent biocompatibility,wide pH-sensitive range of 8.0–4.0,and especially quantitative ability.However,available molecular probes cannot simultaneously meet all the requirements since it is extremely difficult to integrate multiple functionalities into a single molecule.To fully address this issue,we herein integrate two fluorogenic pH sensitive units,a mitochondria-specific block,cellpenetrating facilitator,and biocompatible segments into an elegant silica nano scaffold,which greatly ensures the applicability for real-time tracking of pH fluctuations in mitophagy.Most significantly,at a single wavelength excitation,the integrated pHsensitive units have spectra-distinguishable fluorescence towards alkaline and acidic pH in a broad range that covers mitochondrial and lysosomal pH,thus enabling a ratiometric analysis of pH variations during the whole mitophagy.This work also provides constructive insights into the fabrication of advanced fluorescent nanoprobes for diverse biomedical applications.
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