Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level

作     者：Fengjiao Zhu Yahui Ji Jiu Deng Linmei Li Xue Bai Xianming Liu Bingcheng Lin Yao Lu Fengjiao Zhu;Yahui Ji;Jiu Deng;Linmei Li;Xue Bai;Xianming Liu;Bingcheng Lin;Yao Lu

作者机构：Department of BiotechnologyDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第6期

页      面：2893-2900页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 0703[理学-化学] 

基　　金：the National Natural Science Foundation of China(Nos.21874133,31927802) Youth Innovation Promotion Association CAS(No.2018217) funds from the Dalian Institute of Chemical Physics,CAS(No.I201908)。 

主　　题：Microfluidics Single-cell EV analysis Single EV analysis Extracellular vesicle Heterogeneity 

摘      要：Extracellular vesicles(EVs)are membrane vesicles secreted by cells,playing critical roles in mediating intercellular communications for various physiological and pathological processes.Most of the EV analysis is currently performed at the bulk level,obscuring the origin of the EVs and diverse characteristics of the individual extracellular vesicle.Technologies to analyze the extracellular vesicles at the single-cell and single-vesicle levels are needed to evaluate EV comprehensively and decode the heterogeneity underlying EV secretion.Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims.Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis,which would be summarized in this mini-review.