Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies

作     者：Suying Liu Jiaofang Huang Chen Zhang Lihua Wang Chunhai Fan Chao Zhong 

作者机构：Division of Physical Biology and Bioimaging CenterShanghai Institute of Applied PhysicsChinese Academy of SciencesShanghaiChina University of Chinese Academy of SciencesBeijingChina School of Physical Science and TechnologyShanghaiTech UniversityShanghaiChina State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina Institute of PhysicsSwiss Federal Institute of Technology Lausanne(EPFL)LausanneSwitzerland The Interdisciplinary Research CenterShanghai Advanced Research InstituteChinese Academy of SciencesZhangjiang LaboratoryShanghaiChina School of Chemistry and Chemical EngineeringInstitute of Molecular MedicineRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Center for Materials Synthetic BiologyShenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina CAS Key Laboratory of Quantitative Engineering BiologyShenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2022年第7卷第3期

页      面：965-971页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：funded by the National Key R&D Program of China(Nos.2020YFA0908100 and 2021YFA0910800) the National Science Fund for Distinguished Young Scholars(No.32125023) supported by grants from the National Natural Science Foundation of China(No.31872728) the National Science and Technology Major Project of the Ministry of Science and Technology of China(2020YFA0908900) the Science and Technology Commission of Shanghai Municipality(Nos.19ZR1477100 and 22ZR1416000)for J.F.Huang 

主　　题：Bacillus subtilis Biofilms Synthetic biology Living materials Environmental tolerance 

摘      要：Bacterial communities form biofilms on various surfaces by synthesizing a cohesive and protective extracellular matrix,and these biofilms protect microorganisms against harsh environmental *** subtilis is a widely used experimental species,and its biofilms are used as representative models of beneficial ***,*** biofilms are known to be rich in extracellular polymeric substances(EPS)and other biopolymers such as DNA and proteins like the amyloid protein TasA and the hydrophobic protein *** materials,which form an interconnected,cohesive,three-dimensional polymer network,provide the mechanical stability of biofilms and mediate their adherence to surfaces among other functional ***,we explored how genetically-encoded components specifically contribute to regulate the growth status,mechanical properties,and antibiotic resistance of *** biofilms,thereby establishing a solid empirical basis for understanding how various genetic engineering efforts are likely to affect the structure and function of *** noted discrete contributions to biofilm morphology,mechanical properties,and survival from major biofilm components such as EPS,TasA and *** example,EPS plays an important role in maintaining the stability of the mechanical properties and the antibiotic resistance of biofilms,whereas BslA has a significant impact on the resolution that can be obtained for printing *** work provides a deeper understanding of the internal interactions of biofilm components through systematic genetic *** thus not only broadens the application prospects of beneficial biofilms,but also serves as the basis of future strategies for targeting and effectively removing harmful biofilms.