Genomic analysis of isopentenyltransferase genes and functional characterization of TaIPT8 indicates positive effects of cytokinins on drought tolerance in wheat

作     者：Na Wang Jun Chen Yuan Gao Yongbin Zhou Ming Chen Zhaoshi Xu Zhengwu Fang Youzhi Ma Na Wang;Jun Chen;Yuan Gao;Yongbin Zhou;Ming Chen;Zhaoshi Xu;Zhengwu Fang;Youzhi Ma

作者机构：Hubei Collaborative Innovation Center for Grain Industry/College of AgricultureYangtze UniversityJingzhou 434000HubeiChina Institute of Crop SciencesChinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic ImprovementKey Laboratory of Biology and Genetic Improvement of Triticeae CropsMinistry of AgricultureBeijing 100081China 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2023年第11卷第1期

页      面：46-56页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：financially supported by the National Key Research and Development Program of China(2020YFE0202300) the Agricultural Science and Technology Innovation Program(CAASZDRW202109 and CAAS-ZDRW202002)。 

主　　题：TaIPT Phytohormone Abiotic stress Cytokinin biosynthesis Wheat 

摘      要：Cytokinins(CKs)function in plant development and during stress responses,but their role in drought tolerance in wheat is unknown.In the present study,24 isopentenyltransferase(IPT)genes,encoding ratelimiting enzymes in CK biosynthesis were identified in the wheat genome.The chromosomal locations and structures of the genes,protein properties,and phylogenetic relationships were characterized.ATP/ADP TaIPT genes showed tissue-specific expression.TaIPT2,TaIPT7,and TaIPT8 expression was rapidly induced by 0.5–1 h drought treatments,which decreased to low levels after 2 h drought treatment,as did most other TaIPT genes.TaIPT8-5a/5b/5d triple mutants showed decreased levels of tZtype CK under normal and drought conditions and reduced drought tolerance,which,however,did not manifest as phenotype alterations.By contrast,transgenic wheat plants with drought-induced TaIPT8showed increased drought tolerance.Our study provides a foundation for further investigation of TaIPT genes and novel insights into the role of CKs in the drought response of wheat.