A novel mouse model simulating transurethral laser vaporization prostatectomy
作者机构：Medical School of Guizhou UniversityGuiyang 550000China Department of Urology SurgeryGuizhou Province People’s HospitalGuiyang 550002China School of Clinical MedicineGuizhou Medical UniversityGuiyang 55025China School of Clinical MedicineGuangzhou Medical UniversityGuangzhou 510180China Department of UrologyShanghai First People’s HospitalShanghai Jiao Tong UniversityShanghai 201620China
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2022年第24卷第2期
页 面：191-194页
核心收录：
基 金：The present study is supported financially by grants from the National Natural Science Foundation of China(No.81860141) the Foundation of Health and Family Planning Commission of Guizhou Province(Gzwjkj-2017-1-032) Guizhou Science-Technology Support Plan Projects(2797).
主 题：animal model benign prostatic hyperplasia transurethral resection of the prostate
摘 要：Benign prostatic hyperplasia(BPH)is a common disease in elderly men,and transurethral laser prostatectomy(TULP)has been widely used in the clinic to remove bladder outlet obstruction caused by BPH.Previous animal models for wound repair after prostatectomy have many limitations,and there have been no previous reports of a mouse model of TULP.Therefore,this study aimed to establish a novel mouse model of TULP.Twelve healthy adult Kunming(KM)mice received transurethral laser vaporization prostatectomy with a 200-μm thulium laser.The mice were sacrificed,and wound specimens from the prostatic urethra and bladder neck were harvested at 1 day,3 days,5 days,and 7 days after surgery.Hematoxylin-eosin(HE)and immunohistochemistry were applied to confirm the establishment of the mouse TULP model.One day after the surgery,urothelium expressing uroplakin(UPK)was absent in the urethral wound site,and a large number of necrotic tissues were found in the wound site.There was no UPK-positive urothelium in the wound 3 days after surgery.At 5 days after surgery,monolayer urothelium expressing UPK was found in the wound site,indicating that the re-epithelization of the wound had been completed.On the 7th day after surgery,there were multiple layers of urothelium with UPK expression,indicating that the repair was completed.It is feasible to establish a mouse TULP model by using a microcystoscope system and a 200-μm thulium laser.
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