Embedding MSCs in Si-HPMC hydrogel decreased MSC-directed host immune response and increased the regenerative potential of macrophages

作     者：Christelle Demarquay Lara Moussa Gildas Réethorée Fabien Milliat Pierre Weiss Noëlle Mathieu 

作者机构：Human Health DepartmentIRSNFrench Institute for Radiological Protection and Nuclear SafetySERAMEDLRMedFontenay-aux-Roses 92262France Facultéde Chirurgie DentaireRegenerative Medicine and Skeleton(RMeS)LaboratoryUniversite de NantesNantes 44042France 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2022年第9卷第1期

页      面：490-500页

核心收录：

学科分类：0710[理学-生物学] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0835[工学-软件工程] 0703[理学-化学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by the French‘Agence Nationale pour le Recherche’(Anthos project-ANR13-RPIB-008) 

主　　题：Si-HPMC hydrogel mesenchymal stromal cells host immune response regenerative medicine 

摘      要：Embedding mesenchymal stromal cells(MSCs)in biomaterial is a subject of increasing interest in the field of Regenerative *** up the clinical use of MSCs is dependent on the use of non-syngeneic models in accordance with Good Manufacturing Practices(GMP)requirements and on *** this end,in this study,we analyzed the in vivo host immune response following local injection of silanized hydroxypropyl methylcellulose(Si-HPMC)-embedded human MSCs in a rat model developing colorectal damage induced by ionizing *** and lymphocytes from mesenteric lymph nodes were harvested in addition to colonic *** set up tests,using flow cytometry and a live imaging system,to highlight the response to specific antibodies and measure the cytotoxicity of lymphocytes against injected *** demonstrated that Si-HPMC protects MSCs from specific antibodies production and from apoptosis by *** also observed that Si-HPMC does not modify innate immune response infiltrate in vivo,and that in vitro co-culture of Si-HPMC-embedded MSCs impacts macrophage inflammatory response depending on the microenvironment but,more importantly,increases the macrophage regenerative response through Wnt-family and VEGF gene *** study furthers our understanding of the mechanisms involved,with a view to improving the therapeutic benefits of biomaterial-assisted cell therapy by modulating the host immune *** decrease in specific immune response against injected MSCs protected by Si-HPMC also opens up new possibilities for allogeneic clinical use.