Identification and dipeptidyl peptidase Ⅳ(DPP-Ⅳ) inhibitory activity verification of peptides from mouse lymphocytes

作     者：Juan Wang Yujia Xie Yuanyuan Luan Tingting Guo Shanshan Xiao Xingxing Zeng Shaohui Zhang Juan Wang;Yujia Xie;Yuanyuan Luan;Tingting Guo;Shanshan Xiao;Xingxing Zeng;Shaohui Zhang

作者机构：School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Zhejiang Go Peptides Life Science and Healthcare Technology Co.Ltd.Wenzhou 325000China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第6期

页      面：1515-1526页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100403[医学-营养与食品卫生学] 10[医学] 

基　　金：(B)Chemistry/Metallurgy/Environment/Mine Industry (E)Medicine&Public Health。 

主　　题：Lymphocytes Peptides Lipopolysaccharide Dipeptidyl peptidaseⅣ(DPP-Ⅳ)inhibitory activity 

摘      要：The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide(LPS)stimulation,to explore novel peptides and to research the bioactive function.Mouse spleen lymphocytes were isolated and cultured with LPS stimulation(experimental group)or not(control group)to collect intracellular peptides.Totally 385 peptides were analyzed by nanoliter liquid phase-Q Exactive quadrupole ultra-high resolution orbitrap mass spectrometer(Nano LC-Q Exactive Plus)and identifi ed by PEAKS X software.After compared with peptides reported,131 novel peptides were discovered,which then were predicted bioactivity by Peptide Ranker and 6 peptides with high bioactivity were predicted function by BIOPEP-UMW database.Prediction data showed that they may have dipeptidyl peptidase IV(DPP-IV)inhibitory activity.Finally,two peptides showed better potent inhibition were verifi ed with competitive and noncompetitive modes.