Distinct neuronal excitability alterations of medial prefrontal cortex in early-life neglect model of rats

作     者：Yu Zhang Xiuping Sun Changsong Dou Xianglei Li Ling Zhang Chuan Qin Yu Zhang;Xiuping Sun;Changsong Dou;Xianglei Li;Ling Zhang;Chuan Qin

作者机构：NHC Key Laboratory of Human Disease Comparative MedicineInstitute of Laboratory Animal SciencesChinese Academy of Medical Sciences(CAMS)Comparative Medicine CenterPeking Union Medical College(PUMC)BeijingChina National Human Diseases Animal Model Resource CenterBeijingChina Beijing Engineering Research Center for Experimental Animal Models of Human Critical DiseasesBeijingChina International Center for Technology and Innovation of animal modelBeijingChina Changping National laboratory(CPNL)BeijingChina 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2022年第5卷第3期

页      面：274-280页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：CAMS Innovation Fund for Medical Sciences(CIFMS),Grant/Award Number:2021-I2M-1-034 National Natural Science Foundation of China,Grant/Award Number:31970510 Young Elite Scientist Sponsorship Program by CAST,Grant/Award Number:2019QNRC001。 

主　　题：early-life neglect model GABAergic glutamatergic maternal separation with early weaning medial prefrontal cortex neuronal excitability 

摘      要：Object:Early-life neglect has irreversible emotional effects on the central nervous system.In this work,we aimed to elucidate distinct functional neural changes in me-dial prefrontal cortex(mPFC)of model rats.Methods:Maternal separation with early weaning was used as a rat model of early-life neglect.The excitation of glutamatergic and GABAergic neurons in rat mPFC was recorded and analyzed by whole-cell patch clamp.Results:Glutamatergic and GABAergic neurons of mPFC were distinguished by typi-cal electrophysiological properties.The excitation of mPFC glutamatergic neurons was significantly increased in male groups,while the excitation of mPFC GABAergic neurons was significant in both female and male groups,but mainly in terms of rest membrane potential and amplitude,respectively.Conclusions:Glutamatergic and GABAergic neurons in medial prefrontal cortex showed different excitability changes in a rat model of early-life neglect,which can contribute to distinct mechanisms for emotional and cognitive manifestations.