Cyclic arginine-glycine-aspartic acid-modified red blood cells for drug delivery:Synthesis and in vitro evaluation
作者机构：Department of PharmaceuticsSchool of PharmacyXiamen Medical CollegeXiamenFujian361023China Institute of Respiratory DiseasesXiamen Medical CollegeXiamenFujian361023China Department of PharmaceuticsSchool of PharmacyHarbin University of CommerceHarbin150076China Department of PharmacyXiamen Xianyue HospitalXiamenFujian361012China
出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)
年 卷 期：2022年第12卷第2期
页 面：324-331页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：support provided by the General Program of the Natural Science Foundation of Fujian Province of China(Grant No.:2019D016) the Program of the Institute of Respiratory Diseases of Xiamen Medical College(Program No.:HXJB-04) the New Century Excellent Talent Support Program of Higher Education Institutions of Fujian Province(Program No.:MinJiaoKe47) the Innovation and Entrepreneurship Training Program for College Students(Program No.:201912631017).
主 题：Cytotoxicity Red blood cells Drug delivery Targeting
摘 要：Red blood cells(RBCs)are an excellent choice for cell preparation research because of their biocompatibility,high drug loading,and long half-life.In this study,doxorubicin(DOX)was encapsulated with RBCs as the carrier.The biotin-avidin system binding principle was used to modify biotinylated cyclic arginine-glycine-aspartic acid(cRGD)onto RBC surfaces for accurate targeting,high drug loading,and sustained drug release.The RBC drug delivery system(DDS)was characterized,and the concentration of surface sulfur in the energy spectrum was 6.330%.The physical and chemical properties of RBC DDS were as follows:drug content,0.857 mg/mL;particle size,3339 nm;potential value,12.5 mV;and cumulative release rate,81.35%.There was no significant change in RBC morphology for up to seven days.The results of the targeting and cytotoxicity studies of RBC DDS showed that many RBCs covered the surfaces of U251 cells,and the fluorescence intensity was higher than that of MCF-7 cells.The IC50 value of unmodified drug-loaded RBCs was 2.5 times higher than that of targeted modified drug-loaded RBCs,indicating that the targeting of cancer cells produced satisfactory inhibition.This study confirms that the RBC DDS has the characteristics of accurate targeting,high drug loading,and slow drug release,which increases its likelihood of becoming a clinical cancer treatment in the future.
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