Thalidomide effect in endothelial cell of acute radiation proctitis

作     者：Ki-Tae Kim Hiun-Suk Chae Jin-Soo Kim Hyung-Keun Kim Young-Seok Cho Whang Choi Kyu-Yong Choi Sang-Young Rho Suk-Jin Kang 

作者机构：Department of Radiology College of Medicine The Catholic University of Korea Uijeongbu St. Mary’s Hospital Department of Internal Medicine College of Medicine The Catholic University of Korea Uijeongbu St. Mary's Hospital Department of Internal Medicine College of Medicine The Catholic University of Korea Our Lady of Mercy Hospital Department of Internal Medicine College of Medicine The Catholic University of Korea Uijeongbu St. Mary’s Hospital Department of Pathology College of Medicine The Catholic University of Korea St. Vincent Hospital 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2008年第14卷第30期

页      面：4779-4783页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Radiation proctitis Von Willebrand factor Thrombomodulin Vascular endothelial growth factor Thalidomide 

摘      要：AIM: To determine whether thalidomide prevents microvascular injury in acute radiation proctitis in white rats. METHODS: Fourteen female Wistar rats were used: six in the radiation group, six in the thalidomide group, and two in normal controls. The radiation and thalidomide groups were irradiated at the pelvic area using a single 30 Gy exposure. The thalidomide (150 mg/kg) was injected into the peritoneum for 7 d from the day of irradiation. All animals were sacrificed and the rectums were removed on day 8 after irradiation. The microvessels of resected specimens were immunohistochemically stained with thrombomodulin (TM), von Willebrand Factor (vWF), and vascular endothelial growth factor (VEGF). RESULTS: The microscopic scores did not differ significantly between the radiation and thalidomide groups, but both were higher than in the control group. Expression of TM was significantly lower inthe endothelial cells (EC) of the radiation group than in the control and thalidomide groups (P 0.001). The number of capillaries expressing vWF in the EC was higher in the radiation group (15.3 ± 6.8) than in the control group (3.7 ± 1.7), and the number of capillaries expressing vWF was attenuated by thalidomide (10.8 ± 3.5, P 0.001). The intensity of VEGF expression in capillaries was greater in the radiation group than in the control group and was also attenuated by thalidomide (P = 0.003). CONCLUSION: The mechanisms of acute radiation- induced proctitis in the rats are related to endothelial cell injury of microvessel, which may be attenuated with thalidomide.