Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle

作     者：LI Siying PAN Yangang TENG Honggang SHAN Yuping YANG Guocheng WANG Hongda LI Siying;PAN Yangang;TENG Honggang;SHAN Yuping;YANG Guocheng;WANG Hongda

作者机构：School of Chemistry and Life ScienceAdvanced Institute of Materials ScienceChangchun University of TechnologyChangchun130012P.R.China State Key Laboratory of Electroanalytical ChemistryChangchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022P.R.China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2022年第38卷第3期

页      面：838-842页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 0703[理学-化学] 10[医学] 

基　　金：This work was supported by the Project of the Education Department of Jilin Province,China(No.JJKH20220666KJ) the Talent of Jilin Province Development Fund Project,China(No.2021Y004) National Natural Science Foundation of China(No.21773017). 

主　　题：Rabies virus Entering host cell Dynamic mechanism Atomic force microscopy(AFM) Force tracing 

摘      要：The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals.Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis,the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive.Here,we utilized a novel force tracing technique based on atomic force microscopy(AFM)to record the process of single viral entry into host cell.We revealed that the force of the rabies virus internalization distributed at(65±25)pN,and the time was identified by two peaks with spacings of(237.2±59.1)and(790.3±134.4)ms with the corresponding speed of 0.12 and 0.04µm/s,respectively.Our results provide insight into the effects of viral shape during the endocytosis process.This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.